Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers
Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers
复制标题
DOI：
10.1023/a:1017510331721
复制
发表时间：
2001-01-01
期刊：
影响因子：
1.9
通讯作者：
Jia, JZ
中科院分区：
文献类型：
--
作者：
Ma, JX;Zhou, RH;Jia, JZ
条锈菌(Puccinia striiformis Westend f.sp.)小麦纹枯病(Tritici)是世界上危害最大的小麦病害之一。小麦-簇毛麦6AL.6VS易位系R43、R55、R64和R77是由3个品种杂交而来的，对条锈病和白粉病均具有抗性。R55与感病品种豫麦18杂交，建立了一个F-2群体。R55的抗条锈病由1对显性基因控制，该显性基因独立于位于染色体6VS片段上的抗白粉病基因Pm21，表明抗条锈病基因和Pm21不可能由同一外源片段携带。这个抗条锈病基因被认为是Yr26，最初被认为也位于染色体臂6VS上。对豫麦18×R55的F-2群体进行了群体分离分析和微卫星标记筛选，确定了与Yr26紧密连锁的3个1B微卫星标记Xgwm11、Xgwm18和Xgwm413。Yr26位于Xgwm11/Xgwm18远1.9 cM处，Xgwm413距Xgwm413 3.2 cM。LOD值分别为21和36.5。因此，Yr26位于1B染色体的短臂上。通过系谱、抗性遗传和分子标记分析，对Yr26的起源和分布进行了研究。结果表明，Yr26来自普通小麦，另外3个6AL.6VS易位系R43、R64和R77也携带Yr26。这些基于PCR的微卫星标记对分离群体中Yr26基因的检测是非常有效的，因此可以应用于小麦育种。
Yellow rust (stripe rust), caused by Puccinia striiformis Westend f. sp. tritici, is one of the most devastating diseases of wheat throughout the world. Wheat-Haynaldia villosa 6AL.6VS translocation lines R43, R55, R64 and R77, derived from the cross of three species, carry resistance to both yellow rust and powdery mildew. An F-2 population was established by crossing R55 with the susceptible cultivar Yumai 18. The yellow rust resistance in R55 was controlled by a single dominant gene, which segregated independently of the powdery mildew resistance gene Pm21 located in the chromosome 6VS segment, indicating that the yellow rust resistance gene and Pm21 are unlikely to be carried by the same alien segment. This yellow rust resistance gene was considered to be Yr26, originally thought to be also located in chromosome arm 6VS. Bulked Segregation Analysis and microsatellite primer screens of the population F-2 of Yumai 18 x R55 identified three chromosome 1B microsatellite locus markers, Xgwm11, Xgwm18 and Xgwm413, closely linked to Yr26. Yr26 was placed 1.9 cM distal of Xgwm11/Xgwml8, which in turn were 3.2 cM from Xgwm413. The respective LOD values were 21 and 36.5. Therefore, Yr26 was located in the short arm of chromosome 1B. The origin and distribution of Yr26 was investigated by pedigree, inheritance of resistance and molecular marker analysis. The results indicated that Yr26 came from Triticum turgidum L. Three other 6AL.6VS translocation lines, R43, R64 and R77, also carried Yr26. These PCR-based microsatellite markers were shown to be very effective for the detection of the Yr26 gene in segregating populations and therefore can be applied in wheat breeding.