Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides
Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides
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DOI：
10.1016/s0003-2697(02)00702-9
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发表时间：
2003-04-01
影响因子：
2.9
通讯作者：
Lakowicz, JR
中科院分区：
文献类型：
--
作者：
Malicka, J;Gryczynski, I;Lakowicz, JR
我们检测了Cy3-和cy5标记的寡核苷酸在离银岛膜表面不同距离上的荧光光谱特性。通过生物素化的牛血清白蛋白（BSA）和亲和素交替层来控制到表面的距离，然后结合生物素化的寡核苷酸。在第一个bsa -亲和素层中，荧光增强最大，接近12倍，在第6层中，荧光增强降低到2倍。第一个bsa -亲和素层的最小寿命比无银石英载玻片短约25倍，其中6个bsa -亲和素层的寿命约短2倍。这些结果表明，最大的荧光增强发生在距离银表面约90埃的地方，这个距离很容易通过一层或两层蛋白质获得。(C) 2003 Elsevier Science（美国）版权所有。
We examined the fluorescence spectral properties of Cy3- and Cy5-labeled oligonucleotides at various distances from the surface of silver island films. The distance to the surface was controlled by alternating layers of biotinylated bovine serum albumin (BSA) and avidin, followed by binding of a biotinylated oligonucleotide. The maximum enhancement of fluorescence near a factor of 12 was observed for the first BSA-avidin layer, with the enhancement decreasing to 2-fold for six layers. The minimum lifetimes were observed for the first BSA-avidin layer, and were about 25-fold shorter than on quartz slides without silver, with the lifetimes being about 2-fold shorter for six BSA-avidin layers. These results suggest that maximum fluorescence enhancements occur about 90 Angstrom from the silver surface, a distance readily obtained by one or two layers of proteins. (C) 2003 Elsevier Science (USA). All rights reserved.