Long noncoding RNA CCDC26 as a potential predictor biomarker contributes to tumorigenesis in pancreatic cancer
Long noncoding RNA CCDC26 as a potential predictor biomarker contributes to tumorigenesis in pancreatic cancer
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长非编码 RNA CCDC26 作为潜在的预测生物标志物有助于胰腺癌的肿瘤发生
DOI：
10.1016/j.biopha.2016.06.059
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发表时间：
2016-10-01
影响因子：
7.5
通讯作者：
Jiang, Airen
中科院分区：
文献类型：
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作者：
Peng, Wei;Jiang, Airen
胰腺癌（PC）是全球第四大常见癌症，并且在所有癌症中患者存活率最低。新的研究表明，长链非编码RNA（lncRNA）存在于癌症患者中，并显示出作为强大的标记物和治疗靶点的巨大潜力。然而，对lncRNA在PC中的作用知之甚少。本研究旨在探讨lncRNA CCDC26（CCDC26）在PC中的表达模式、临床意义及生物学功能。采用实时荧光定量PCR技术，我们分析了40例PC患者中CCDC26的表达水平。我们发现CCDC26在PC组织中的表达显著高于正常组织。CCDC 26水平与肿瘤大小、肿瘤数量和总生存期（OS）降低相关。单因素和多因素分析显示，CCDC26表达是PC患者OS的独立预后因素。CCDC 26的ROCAUC高达0.663，提示CCDC 26可作为PC与正常人的鉴别诊断指标。通过小干扰RNA敲低CCDC26表达显著促进生长停滞和凋亡。CCDC26的表达与PCNA、Bcl 2呈正相关。我们的数据表明，CCDC 26可能是一个新的癌基因在PC中，并负责肿瘤细胞的生长和凋亡，部分通过调节PCNA和Bcl 2的表达。本研究为今后肿瘤临床提供了一种新的PC生物标志物和治疗靶点。(C)2016 Elsevier Masson SAS。All rights reserved.
Pancreatic cancer (PC) is the fourth most common cancer worldwide and has the least patient survival rate of any cancer. Emerging studies have demonstrated that long noncoding RNAs (lncRNAs) were present in cancer patients and have shown great potential as powerful markers and therapeutic targets. However, little is known about the role of lncRNAs in PC. The present study aimed to investigate the expression pattern, clinical significance and biological function of lncRNA CCDC26 (CCDC26) in PC. With quantitative real-time PCR, we analyzed CCDC26 expression levels in 40 PC patients. We found that the CCDC26 expression was significantly higher in PC tissues than in normal tissues. CCDC26 levels were correlated with tumor size, tumor number, and reduced overall survival (OS). Univariate and multivariate analysis showed that CCDC26 expression is an independent prognostic factor of OS in patients with PC. Additionally, ROCAUC of CCDC26 was up to 0.663, implicating that CCDC26 could be a diagnostic marker for distinguishing PC from normal. Knockdown of CCDC26 expression by small interfering RNA significantly promoted growth arrest and apoptosis. Moreover, we found that the expression of CCDC26 was positively correlated with PCNA and Bcl2. Our data suggest that CCDC26 may be identified as a novel oncogene in PC, and responsible for growth and apoptosis of cancer cell, partly by regulating the PCNA and Bcl2 expression. This work provides a novel biomarker and therapeutic target of PC for cancer clinic in future. (C) 2016 Elsevier Masson SAS. All rights reserved.