Inhibitory Effects of Chemical Constituents fromActinidia kolomiktaon LPS-Induced Inflammatory Responses
Inhibitory Effects of Chemical Constituents fromActinidia kolomiktaon LPS-Induced Inflammatory Responses
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猕猴桃化学成分对 LPS 诱导的炎症反应的抑制作用
DOI：
10.1007/s43450-020-00004-w
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Li Gao
中科院分区：
文献类型：
--
作者：
Ye Chao;Jin Mei;Jin Chunshi;Jin Lan;Sun Jinfeng;Ma Ying Jie;Zhou Wei;Li Gao
从猕猴桃科猕猴桃的根茎中分离得到 16 种已知化合物，包括 3 种木脂素苷 (1-3)、7 种酚苷 (4-10)、3 种苷 (11-13) 和 3 种黄酮类化合物 (14-16)。通过广泛的光谱分析并与文献报道的比较，阐明了它们的结构。这项研究首次证实了从猕猴桃科中分离出来的 12 种化合物 (1-4,6-13) 的存在。评估了分离的化合物对 LPS 诱导的 RAW264.7 细胞中 NO、TNF-α 和 IL-6 产生的抑制作用。其中，红景天苷（9）、D-苏型愈创木酰甘油7-O-β-D-吡喃葡萄糖苷（10）、andepi-afzelechin（15）对NO、TNF-α和IL-6表现出有效的抗炎作用。此外，化合物10在50μM时对COX-2的表达表现出显着的抑制作用，化合物15在50μM时对iNOS的表达表现出显着的抑制作用。这些结果表明A.的抗炎活性。 kolomiktam 可能部分通过抑制促炎细胞因子的表达而发生。
Sixteen known compounds, including three lignan glycosides (1–3), seven phenolic glycosides (4–10), three glycosides (11–13), and three flavonoids (14–16), were isolated from the rhizomes ofActinidia kolomikta(Rupr. & Maxim.) Maxim., Actinidiaceae. Their structures were elucidated through extensive spectroscopic analyses and comparison with those reported in the literature. This study is the first confirmation of the presence of the twelve compounds (1–4,6–13) isolated from the Actinidiaceae family. The isolated compounds were evaluated for their inhibitory effects on NO, TNF-α, and IL-6 production in LPS-induced RAW264.7 cells. Among them, salidroside (9),D-threo-guaiacylglycerol 7-O-β-D-glucopyranoside (10), andepi-afzelechin (15) exhibited the potent anti-inflammatory effect against NO, TNF-α, and IL-6. In addition, compound10showed significant inhibitory effects on COX-2 expression at 50 μM, and compound15showed significant inhibitory effects on iNOS expression at 50 μM. These results suggest that the anti-inflammatory activity ofA. kolomiktamight in part occur via the inhibition of the expression of the pro-inflammatory cytokines.