The Geobacillus stearothermophilus V iscS gene, encoding cysteine desulfurase, confers resistance to potassium tellurite in Escherichia coli K-12.
The Geobacillus stearothermophilus V iscS gene, encoding cysteine desulfurase, confers resistance to potassium tellurite in Escherichia coli K-12.
复制标题
嗜热脂肪地芽孢杆菌 V iscS 基因编码半胱氨酸脱硫酶，赋予大肠杆菌 K-12 对亚碲酸钾的抗性。
DOI：
10.1128/jb.185.19.5831-5837.2003
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发表时间：
2003
影响因子：
3.2
通讯作者：
Vásquez,ClaudioC
中科院分区：
文献类型：
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作者：
Tantaleán,JuanC;Araya,ManuelA;Saavedra,ClaudiaP;Fuentes,DerieE;Pérez,JoséM;Calderón,IvánL;Youderian,Philip;Vásquez,ClaudioC
许多真细菌对有毒氧化剂亚碲酸钾具有抗性，而亚碲酸钾抗性涉及多种生化机制。来自对亚碲酸盐具有天然抗性的嗜热脂肪地芽孢杆菌 V 的 theiscS 基因的表达赋予对亚碲酸盐天然敏感的大肠杆菌 K-12 具有亚碲酸盐抗性。 TheG.嗜热脂肪菌 iscSgene 编码半胱氨酸脱硫酶。阻止其磷酸吡哆醛辅因子结合的定点突变 iniscS 消除了酶活性及其赋予大肠杆菌亚碲酸盐抗性的能力。大肠杆菌。 G 的表达嗜热脂肪菌 iscSgene 赋予亚碲酸盐过敏性大肠杆菌抗亚碲酸盐性。大肠杆菌 iscSandsodA sodB 突变体（超氧化物歧化酶缺陷）并补充 anE 的营养缺陷型需求。大肠杆菌是硫胺素突变体，但不是烟酸突变体。这些和其他结果支持这样的假设：亚碲酸盐的还原产生超氧阴离子，并且超氧化物损伤的主要目标是大肠杆菌。带有铁硫簇的大肠杆菌酶。
Many eubacteria are resistant to the toxic oxidizing agent potassium tellurite, and tellurite resistance involves diverse biochemical mechanisms. Expression of theiscSgene fromGeobacillus stearothermophilusV, which is naturally resistant to tellurite, confers tellurite resistance inEscherichia coliK-12, which is naturally sensitive to tellurite. TheG. stearothermophilus iscSgene encodes a cysteine desulfurase. A site-directed mutation iniscSthat prevents binding of its pyridoxal phosphate cofactor abolishes both enzyme activity and its ability to confer tellurite resistance inE. coli. Expression of theG. stearothermophilus iscSgene confers tellurite resistance in tellurite-hypersensitiveE. coli iscSandsodA sodBmutants (deficient in superoxide dismutase) and complements the auxotrophic requirement of anE. coli iscSmutant for thiamine but not for nicotinic acid. These and other results support the hypothesis that the reduction of tellurite generates superoxide anions and that the primary targets of superoxide damage inE. coliare enzymes with iron-sulfur clusters.