IDENTIFICATION AND LOCALIZATION OF IMMUNOREACTIVE FORMS OF CALDESMON IN SMOOTH AND NONMUSCLE CELLS - A COMPARISON WITH THE DISTRIBUTIONS OF TROPOMYOSIN AND ALPHA-ACTININ
IDENTIFICATION AND LOCALIZATION OF IMMUNOREACTIVE FORMS OF CALDESMON IN SMOOTH AND NONMUSCLE CELLS - A COMPARISON WITH THE DISTRIBUTIONS OF TROPOMYOSIN AND ALPHA-ACTININ
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DOI：
10.1083/jcb.100.5.1656
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发表时间：
1985-01-01
影响因子：
7.8
通讯作者：
LYNCH, W
中科院分区：
文献类型：
--
作者：
BRETSCHER, A;LYNCH, W
Caldesmon是鸡肌砂囊平滑肌中的一种F-肌动蛋白交联蛋白，其F-肌动蛋白结合活性在体外可受Ca ~（2+）-钙调蛋白调节。它是一种杆状、热稳定的F-肌动蛋白捆绑蛋白，是目前已知的鸡砂囊平滑肌中最丰富的F-肌动蛋白交联蛋白。钙调素的多克隆抗体用于研究其在其他细胞中的分布和定位。免疫印迹程序，在培养的细胞中检测到免疫反应性的热稳定形式的钙调蛋白，其具有与肌胃钙调蛋白大致相同的表观多肽MW（120，000 - 140，000）或显著更低的MW（71，000 - 77，000）。通过使用亲和纯化的抗体在间接免疫荧光显微镜，免疫反应性形式被定位到末端网的刷状缘的肠上皮细胞和应力纤维和皱褶膜的培养细胞。在光学显微镜水平下，钙调素以周期性方式沿着应力纤维分布，其与原肌球蛋白的分布一致并与α-肌球蛋白的分布互补。辅肌动蛋白
Caldesmon is an F-actin cross-linking protein of chicken gizzard smooth muscle whose F-actin binding activity can be regulated in vitro by Ca2+-calmodulin. It is a rod-shaped, heat-stable, F-actin bundling protein and is the most abundant F-actin cross-linking protein of chicken gizzard smooth muscle presently known. Polyclonal antibodies to caldesmon are used to investigate its distribution and localization in other cells. Immune blotting procedures, detected immunoreactive, heat-stable forms of caldesmon in cultured cells having either approximately the same apparent polypeptide MW as gizzard caldesmon (120,000-140,000) or a substantially lower MW (71,000-77,000). Through use of affinity-purified antibodies in indirect immunofluorescence microscopy, the immunoreactive forms were localized to the terminal web of the brush border of intestinal epithelial cells and to the stress fibers and ruffling membranes of cultured cells. At the light microscope level caldesmon is distributed in a periodic fashion along stress fibers that is coincident with the distribution of tropomyosin and complementary to the distribution of .alpha.-actinin.