Chronic ZnO-NPs exposure at environmentally relevant concentrations results in metabolic and locomotive toxicities in Caenorhabditis elegans
Chronic ZnO-NPs exposure at environmentally relevant concentrations results in metabolic and locomotive toxicities in Caenorhabditis elegans
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DOI：
10.1016/j.envpol.2016.10.086
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发表时间：
2017-01-01
影响因子：
8.9
通讯作者：
Liao, Vivian Hsiu-Chuan
中科院分区：
文献类型：
--
作者：
Huang, Chi-Wei;Li, Shang-Wei;Liao, Vivian Hsiu-Chuan
氧化锌纳米颗粒（ZnO- nps）是一种新兴的污染物，引起了人们对水生环境潜在风险的关注。经测算，水体环境ZnO-NPs浓度为76 μ g/l。我们的目的是利用秀丽隐杆线虫确定在环境相关浓度下长期暴露ZnO-NPs的水生毒性。两种模拟环境相关介质-中硬重构水（EPA水）和模拟土壤孔隙水(SSPW)-分别代表沉积物中的地表水和孔隙水。结果表明，EPA水体中ZnO-NPs的水动力直径比SSPW水体小得多。尽管在两种培养基中Zn离子释放量随时间的增加而增加，但在48 h和72 h， EPA水中Zn离子浓度比SSPW中增加了两倍。ZnO-NPs在两种培养基中都没有引起线虫的生长缺陷或减少头部抽打。然而，即使在相对较低浓度（0.05 μ g/l）的EPA水中，长期暴露于ZnO-NPs也会导致秀丽隐杆线虫体弯曲显著减少；在SSPW中没有观察到类似的结果。此外，在相同浓度（50和500 μ g/l）下，ZnO-NPs比ZnCl2在EPA水中更严重地减少了秀丽隐杆线虫的体弯曲。在EPA水中暴露ZnO-NPs（50和500 μ g/l）后，ATP水平持续显著降低，并诱导活性氧。我们的研究结果提供了证据，证明在环境相关浓度下长期暴露于ZnO-NPs会引起代谢和运动毒性，这意味着低浓度的ZnO-NPs在水生环境中具有潜在的生态毒性。(C) 2016 Elsevier Ltd.版权所有。
ZnO nanoparticles (ZnO-NPs) are emerging contaminants that raise the concerns of potential risk in the aquatic environment. It has been estimated that the environmental ZnO-NPs concentration is 76 mu g/l in the aquatic environment. Our aim was to determine the aquatic toxicity of ZnO-NPs with chronic exposure at environmentally relevant concentrations using the nematode Caenorhabditis elegans. Two simulated environmentally relevant mediums-moderately hard reconstituted water (EPA water) and simulated soil pore water (SSPW)-were used to represent surface water and pore water in sediment, respectively. The results showed that the ZnO-NPs in EPA water has a much smaller hydrodynamic diameter than that in SSPW. Although the ionic release of Zn ions increased time-dependently in both mediums, the Zn ions concentrations in EPA water increased two-fold more than that in SSPW at 48 h and 72 h. The ZnO-NPs did not induce growth defects or decrease head thrashes in C elegans in either media. However, chronic exposure to ZnO-NPs caused a significant reduction in C elegans body bends in EPA water even with a relatively low concentration (0.05 mu g/l); similar results were not observed in SSPW. Moreover, at the same concentrations (50 and 500 mu g/l), body bends in C. elegans were reduced more severely in ZnO-NPs than in ZnCl2 in EPA water. The ATP levels were consistently and significantly decreased, and ROS was induced after ZnO-NPs exposure (50 and 500 mu g/l) in EPA water. Our results provide evidences that chronic exposure to ZnO-NPs under environmentally relevant concentrations causes metabolic and locomotive toxicities implicating the potential ecotoxicity of ZnO-NPs at low concentrations in aquatic environments. (C) 2016 Elsevier Ltd. All rights reserved.