Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality.
Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality.
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DOI：
10.1091/mbc.5.10.1145
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发表时间：
1994-10
影响因子：
3.3
通讯作者：
Kohta Takahashi;Hiroshi Yamada;M. Yanagida
中科院分区：
文献类型：
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作者：
Kohta Takahashi;Hiroshi Yamada;M. Yanagida
细胞分裂周期中染色体的精确传递是由许多基因维持的。在本研究中所做的尝试是分离温度敏感（ts）裂殖酵母突变体，显示在允许或半允许温度（指定为mis）的微型染色体的高损失率。通过对539株含有微染色体的ts菌株的菌落颜色测定，我们鉴定出12个遗传位点（mis 1-mis 12），并确定了它们在限制性温度下的表型。其中7个与限制性温度下的细胞周期阻滞表型有关，其中3个与有丝分裂有关。在mis 6和mis 12中，子体中规则染色体的不均匀分布是广泛的。细胞在细胞分裂后由于错误分离而变得不活动。mis 5的表型为DNA复制缺陷型，对紫外线和羟基脲过敏。mis 5+编码复制开始所需的普遍存在的MCM家族的新成员。mis 5+基因对于存活力是必需的，并且在功能上不同于先前在裂殖酵母中鉴定的其他成员cdc 21+、nda 1+和nda 4+。mis 11突变体表型是细胞分裂阻滞，细胞大小减小。在mis 11中，G1和G2期的进展被阻断。克隆的mis 11+基因与prp 2+相同，prp 2+是RNA剪接所必需的，与哺乳动物剪接因子U2 AF 65相似。
Precise chromosome transmission in cell division cycle is maintained by a number of genes. The attempt made in the present study was to isolate temperature-sensitive (ts) fission yeast mutants that display high loss rates of minichromosomes at permissive or semipermissive temperature (designated mis). By colony color assay of 539 ts strains that contain a minichromosome, we have identified 12 genetic loci (mis1-mis12) and determined their phenotypes at restrictive temperature. Seven of them are related to cell cycle block phenotype at restrictive temperature, three of them in mitosis. Unequal distribution of regular chromosomes in the daughters is extensive in mis6 and mis12. Cells become inviable after rounds of cell division due to missegregation. The phenotype of mis5 is DNA replication defect and hypersensitivity to UV ray and hydroxyurea. mis5+ encodes a novel member of the ubiquitous MCM family required for the onset of replication. The mis5+ gene is essential for viability and functionally distinct from other previously identified members in fission yeast, cdc21+, nda1+, and nda4+. The mis11 mutant phenotype was the cell division block with reduced cell size. Progression of the G1 and G2 phases is blocked in mis11. The cloned mis11+ gene is identical to prp2+, which is essential for RNA splicing and similar to a mammalian splicing factor U2AF65.