Proliferative responses of rat nasal epithelia to ozone.
Proliferative responses of rat nasal epithelia to ozone.
复制标题
大鼠鼻上皮对臭氧的增殖反应。
DOI：
10.1016/0041-008x(90)90270-5
复制
发表时间：
1990
影响因子：
3.8
通讯作者：
Henderson,RF
中科院分区：
文献类型：
--
作者：
Johnson,NF;Hotchkiss,JA;Harkema,JR;Henderson,RF
对雌性Fischer 344N大鼠前鼻腔上皮进行了观察，发现前鼻腔上皮由四种类型的上皮组成：鳞状上皮、纤毛呼吸上皮、无纤毛立方/移行上皮和嗅觉上皮。用溴脱氧尿苷(BrdUrd)掺入法检测DNA在这些组织中的复制。用识别BrdUrd的单抗鉴定标记细胞。对照动物的纤毛呼吸道、非纤毛立方/移行和嗅觉上皮DNA复制水平较低(1个标记细胞/mm基板)，而鳞状上皮每毫米基板含有40个标记细胞。雌性Fischer 344 N大鼠暴露在0.0、0.12、0.27或0.8ppm臭氧中，每天6小时，最多7天。观察是在暴露后3天或7天以及从7天暴露中恢复3天或7天后进行的。暴露于0.8ppm臭氧后，在无纤毛的立方/移行细胞中，DNA复制出现了短暂但显著的增加，而在有纤毛的呼吸和嗅觉上皮细胞中，DNA复制的一过性增加没有那么明显。这种增加在暴露3天后显着，暴露7天时，除立方/移行上皮外，其余均消失。暴露在0.8ppm臭氧中3天或7天都会导致立方体上皮的增生。在臭氧暴露到0.8ppm的7天后，在7天的恢复后，鳞状上皮中的DNA复制水平被抑制。这项研究表明，在大鼠的前鼻上皮内，DNA复制存在区域性差异，这些水平受到刺激物的影响。立方/移行上皮是对臭氧暴露影响最敏感的上皮细胞类型，因此可以提供呼吸道刺激性损害的敏感指标。
The epithelium of the female Fischer 344 N rat anterior nasal cavity was examined and found to be composed of four types of epithelia: squamous, ciliated respiratory, nonciliated cuboidal/transitional, and olfactory. DNA replication in these tissues was monitored by bromodeoxyuridine (BrdUrd) incorporation. Labeled cells were identified using a monoclonal antibody recognizing BrdUrd. Ciliated respiratory, nonciliated cuboidal/transitional, and olfactory epithelia from control animals had a low level of DNA replication (1 labeled cell/mm basal lamina); in contrast, the squamous epithelium contained 40 labeled cells per millimeter basal lamina. Female Fischer 344 N rats were exposed to 0.0, 0.12, 0.27, or 0.8 ppm ozone, 6 hr/day for up to 7 days. Observations were made after 3 or 7 days of exposure and after 3 or 7 days of recovery from the 7-day exposure. Following exposure to 0.8 ppm ozone, a transient but marked increase in DNA replication was seen in the nonciliated cuboidal/transitional, while in ciliated respiratory and olfactory epithelia the transient increase in DNA replication was less marked. This increase was prominent after 3 days of exposure and absent by 7 days of exposure in all but the cuboidal/transitional epithelium. Exposure to 0.8 ppm ozone for either 3 or 7 days resulted in hyperplasia of the cuboidal epithelium. A depressed level of DNA replication was seen in the squamous epithelium following 7 days of recovery from 7 days of ozone exposure to 0.8 ppm ozone. This study shows that there are regional differences in DNA replication within the anterior nasal epithelium of the rat and that these levels are modulated by exposure to irritants. The cuboidal/transitional epithelium was the most responsive epithelial cell type to the effects of ozone exposure and may, therefore, provide a sensitive indicator of irritant damage to the respiratory tract.