Exoelectrogenic capacity of host microbiota predicts lymphocyte recruitment to the gut
Exoelectrogenic capacity of host microbiota predicts lymphocyte recruitment to the gut
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DOI：
10.1152/physiolgenomics.00010.2015
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发表时间：
2015-07-01
影响因子：
4.6
通讯作者：
Hagan, Catherine Elizabeth
中科院分区：
文献类型：
--
作者：
Ericsson, Aaron Conrad;Davis, Daniel John;Hagan, Catherine Elizabeth
趋电性，响应于电势的定向细胞运动，已经在包括淋巴细胞在内的广泛的细胞类型中得到证实。外生产电菌，能够产生电流的微生物，已被确定在微生物燃料电池。然而，没有研究调查新鲜粪便中的外生电微生物或外生电微生物群对宿主生物体的影响。在这里，我们表明，肠道微生物种群不同的电流生产的能力，由exoelectrogens和这些差异是预测增加淋巴细胞运输到肠道在体内，尽管缺乏典型的淋巴细胞特异性趋化因子的生产增加。此外，我们证明了从不同商业来源购买的小鼠之间的电流产生的差异与分段丝状细菌的存在或不存在可重复地相关，虽然我们的数据不支持分段丝状细菌在离体电流产生中的直接作用，但外生电微生物群可以通过回肠中存在的粘膜相关细菌在体内转移。此外，我们检测到与细胞外电子转移相关的微生物基因在小鼠粪便中的上调，这些小鼠粪便中定植有含有分节丝状细菌的外生电微生物群。虽然仍然相关，但这些结果表明了肠道微生物群调节免疫系统细胞向肠道募集的新方法。
Electrotaxis, directional cell movement in response to an electric potential, has been demonstrated in a wide range of cell types including lymphocytes. Exoelectrogens, microorganisms capable of generating electrical currents, have been identified in microbial fuel cells. However, no studies have investigated exoelectrogenic microbes in fresh feces or the effects of an exoelectrogenic microbiota on the host organism. Here we show that commensal gut microbial populations differ in their capacity for electrical current production by exoelectrogens and that those differences are predictive of increased lymphocyte trafficking to the gut in vivo, despite the lack of increased production of canonical lymphocyte-specific chemokines. Additionally, we demonstrate that the difference in current production between mice purchased from different commercial sources correlates reproducibly with the presence or absence of segmented filamentous bacteria, and while our data do not support a direct role for segmented filamentous bacteria in ex vivo current production, an exoelectrogenic microbiota can be transferred in vivo via mucosa-associated bacteria present in the ileum. Moreover, we detect upregulation of microbial genes associated with extracellular electron transfer in feces of mice colonized with exoelectrogenic microbiota containing segmented filamentous bacteria. While still correlative, these results suggest a novel means by which the gut microbiota modulates the recruitment of cells of the immune system to the gut.