Glucocorticoid-induced upregulation of proteolipid protein and myelin-associated glycoprotein genes in C6 cells.
Glucocorticoid-induced upregulation of proteolipid protein and myelin-associated glycoprotein genes in C6 cells.
复制标题
糖皮质激素诱导 C6 细胞中蛋白脂质蛋白和髓磷脂相关糖蛋白基因的上调。
DOI：
10.1002/jnr.490370206
复制
发表时间：
1994
影响因子：
4.2
通讯作者：
Konat,GW
中科院分区：
文献类型：
--
作者：
Zhu,W;Wiggins,RC;Konat,GW
研究了地塞米松对大鼠C6胶质瘤细胞中蛋白脂蛋白（PLP）和髓磷脂相关糖蛋白（MAG）基因表达的影响。通过Northern blot分析，定量测定各组mRNA的稳态水平。用地塞米松处理的细胞瞬间上调了这两个基因的表达，PLP基因的mRNA水平峰值在第3天出现，MAG基因的mRNA水平峰值在第5天出现，约为对照水平的10倍。在10−9 ~ 10−5M范围内，与药物浓度直接相关。同时暴露于地塞米松和维甲酸对细胞PLP基因表达有加性影响，而MAG基因表达被抑制在可检测水平以下。基因对地塞米松和维甲酸的不同反应支持了基因受不同机制控制的观点。此外，研究结果表明，地塞米松和维甲酸对髓磷脂基因的影响是通过不同的调控途径介导的。©1994 Wiley‐Liss, Inc。
The effect of dexamethasone on the expression of proteolipid protein (PLP) and myelin‐associated glycoprotein (MAG) genes was investigated in rat C6 glioma cells. The steady state level of the respective mRNA was quantitated by Northern blot analysis. The treatment of cells with dexamethasone transiently upregulated the expression of both genes with peak mRNA levels of approximately 10‐fold over control levels occurring at day 3 for the PLP gene and at day 5 for the MAG gene. The effect was directly related to the drug concentration in the range from 10−9to 10−5M. Combined exposure of the cells to dexamethasone and retinoic acid featured an additive effect on PLP gene expression, whereas MAG gene expression was depressed below detectability level. The dissimilarity in the response of the genes to dexamethasone and retinoic acid supports the contention that the genes are controlled by different mechanisms. Furthermore, the results indicate that the effects of dexamethasone and retinoic acid on the myelin genes are mediated by different regulatory pathways. © 1994 Wiley‐Liss, Inc.