Atrial specific granules of the rat heart: light microscopic staining and histochemical reactions.

Atrial specific granules of the rat heart: light microscopic staining and histochemical reactions.
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大鼠心脏的心房特异性颗粒:光镜染色和组织化学反应。

DOI:
10.1177/26.12.83339
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发表时间:
1978
期刊:
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子:
--
通讯作者:
Raymond
Raymond
中科院分区:
--
文献类型:
--
作者:
J. A.;Bold;J. J.;Raymond

文献摘要

被引文献

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本文对大鼠心房特殊颗粒的一般染色和组织化学性质进行了研究。人们发现,这些颗粒可以证明使用醛品红预处理后,涉及氧化或硫代硫酸化。这种新的方式来证明心房颗粒相比,其他染色方法的灵敏度和选择性,以及性质的反应基团,可能参与染色反应。组织化学未检测到脂质或碳水化合物。反应的总体评估表明,心房颗粒是蛋白质或多肽的储存部位。一些测试表明,这些可能含有色氨酸和含硫氨基酸。
An investigation was carried out to determine general staining and histochemical properties of rat atrial specific granules. It was found that these granules may be demonstrated using aldehyde fuchsin after pretreatments which involve oxidation or thiosulfation. This new way of demonstrating atrial granules is compared to other staining methods in terms of sensitivity and selectivity as well as to the nature of reactive groups that may be involved in the staining reactions. No lipid or carbohydrate were detected histochemically. Overall assessment of reactions suggests that atrial granules are a site of storage for a protein or polypeptide. Some of the tests indicate that these may contain tryptophan and sulfur-containing amino acids.