Simultaneous expression by porcine aorta endothelial cells of glycosphingolipids bearing the major epitope for human xenoreactive antibodies (Gal alpha 1-3Gal), blood group H determinant and N-glycolylneuraminic acid
Simultaneous expression by porcine aorta endothelial cells of glycosphingolipids bearing the major epitope for human xenoreactive antibodies (Gal alpha 1-3Gal), blood group H determinant and N-glycolylneuraminic acid
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DOI：
10.1007/bf01053190
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发表时间：
1996-12-01
影响因子：
3
通讯作者：
Bouhours, JF
中科院分区：
文献类型：
--
作者：
Bouhours, D;Pourcel, C;Bouhours, JF
用c -14-半乳糖或c -14-氨基葡萄糖标记的猪内皮细胞原代培养物中分离出鞘糖脂。它们的特点是在薄层色谱上的迁移性、对外糖苷酶的敏感性和抗体的标记性。除了主要的糖鞘脂，globotetra-和globotriaosylneuroide外，次要的被鉴定为新乳糖系列的五和七糖基神经酰胺，这些神经酰胺由Gal α 1-3Gal-（异反应性表位）或Fuc α 1-2Gal- （H决定因子）终止。发现两种神经节苷类，GM3和GD3， n -糖基神经氨酸是它们的主要唾液酸。因此，猪内皮细胞与人内皮细胞的不同之处在于其表达的鞘糖脂在人体内是不存在的：两种被人天然抗体识别的Gal α 1- 3gal端糖脂，以及两种n -糖基神经氨酸端神经节苷，它们是有效的免疫原。
Glycosphingolipids were isolated from primary cultures of porcine endothelial cells labelled with C-14-galactose or C-14-glucosamine. They were characterized by their mobility on thin layer chromatogram, their sensitivity to exoglycosidases, and their labelling with antibodies. In addition to the major glycosphingolipids, globotetra- and globotriaosylceramide, minor ones were identified as penta- and heptaglycosylceramide of the neolactoseries terminated by either Gal alpha 1-3Gal- (xenoreactive epitope) or Fuc alpha 1-2Gal- (H determinant). Two gangliosides were found, GM3 and GD3, and N-glycolylneuraminic acid was their major sialic acid. Therefore, porcine endothelial cells differ from human endothelial cells by expression of glycosphingolipids that are absent in man: two Gal alpha 1-3Gal-terminated glycolipids recognized by human natural antibodies, and two N-glycolylneuraminic acid-terminated gangliosides which are potent immunogens.