Identification of Drought Tolerance Determinants by Genetic Analysis of Root Response to Drought Stress and Abscisic Acid1
Identification of Drought Tolerance Determinants by Genetic Analysis of Root Response to Drought Stress and Abscisic Acid1
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DOI：
10.1104/pp.106.084632
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发表时间：
2006-09
期刊：
影响因子：
7.4
通讯作者：
L. Xiong;Ruigang Wang;Guohong Mao;J. Koczan
中科院分区：
文献类型：
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作者：
L. Xiong;Ruigang Wang;Guohong Mao;J. Koczan
干旱胁迫是一种常见的严重影响作物产量的不利环境条件。由于干旱作为一种胁迫信号的复杂性，破译干旱耐受机制仍然是植物生物学家面临的主要挑战。为了开发研究植物耐旱性的新方法，我们寻找干旱胁迫赋予的表型，并确定干旱胁迫对侧根发育的抑制是对胁迫的适应性反应。这种干旱反应部分由植物激素脱落酸介导。设计了使用拟南芥（拟南芥）的遗传筛选，并分离出对侧根发育中的干旱或脱落酸的响应改变的侧根生长（dig）突变体的干旱抑制。对这些dig突变体的表征表明，它们也表现出改变的干旱胁迫耐受性，表明这种对干旱胁迫的根反应与整个植物的干旱适应密切相关，并且可以用作获得难以捉摸的耐旱机制的性状。我们的研究还表明，多种机制共存，共同促进全株耐旱性。
Drought stress is a common adverse environmental condition that seriously affects crop productivity worldwide. Due to the complexity of drought as a stress signal, deciphering drought tolerance mechanisms has remained a major challenge to plant biologists. To develop new approaches to study plant drought tolerance, we searched for phenotypes conferred by drought stress and identified the inhibition of lateral root development by drought stress as an adaptive response to the stress. This drought response is partly mediated by the phytohormone abscisic acid. Genetic screens using Arabidopsis (Arabidopsis thaliana) were devised, and drought inhibition of lateral root growth (dig) mutants with altered responses to drought or abscisic acid in lateral root development were isolated. Characterization of these dig mutants revealed that they also exhibit altered drought stress tolerance, indicating that this root response to drought stress is intimately linked to drought adaptation of the entire plant and can be used as a trait to access the elusive drought tolerance machinery. Our study also revealed that multiple mechanisms coexist and together contribute to whole-plant drought tolerance.