Progress on isolation and short-term ex-vivo culture of highly purified non-apoptotic human intestinal epithelial cells (IEC)
Progress on isolation and short-term ex-vivo culture of highly purified non-apoptotic human intestinal epithelial cells (IEC)
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DOI：
10.1078/0171-9335-00312
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发表时间：
2003-05-01
影响因子：
6.6
通讯作者：
Rogler, G
中科院分区：
文献类型：
--
作者：
Grossmann, J;Walther, K;Rogler, G
肠上皮细胞（IEC）形成人体最大的表面，对于消化和吸收营养物质至关重要。此外，这些细胞在保护生物体免受肠腔中存在的微生物和毒素的侵害方面发挥着关键作用。因此，一大批研究人员对这些细胞的研究表现出极大的兴趣也就不足为奇了。然而，迄今为止，纯化活的原代人肠上皮细胞是一个挑战，并且在离体培养物中维持 IEC 更加挑剔，因为在分离过程后，由于细胞锚定的丧失（“失巢凋亡”），IEC 在数小时内就会发生凋亡。近年来，作者致力于不断改进原代 IEC 的分离技术，从而能够简单、有效和快速地分离高度纯化的非凋亡人 IEC。在这项研究中，提出了新改进的方法，并应用于建立高度纯化、完全可行的原代 IEC 的离体培养物，显示出重要的功能特性，使这些细胞适合对原代人肠上皮细胞进行离体研究。
Intestinal epithelial cells (IEC) form the largest surface of the human body and are of pivotal importance to digest and absorb nutrients. Furthermore these cells play a critical role shielding the organism against microorganisms and toxins present in the intestinal lumen. It is therefore not surprising that a large group of researchers take great interest in the study of these cells. However, to date it is a challenge to purify viable primary human intestinal epithelial cells and it has been even more fastidious to maintain IEC in culture ex-vivo as IEC undergo apoptosis within hours due to loss of cell anchorage ('anoikis') following the isolation process.Over recent years the authors aimed to continuously improve the isolation technique for primary IEC, allowing a simple, effective and rapid isolation of highly purified non-apoptotic human IEC. In this study the newly improved method is presented and applied to establish ex-vivo cultures of highly purified, fully viable primary IEC displaying important functional properties, making these cells amenable for ex-vivo research on primary human intestinal epithelial cells.