Cloning and Characterization of an Uncoupling Protein Homolog: A Potential Molecular Mediator of Human Thermogenesis
Cloning and Characterization of an Uncoupling Protein Homolog: A Potential Molecular Mediator of Human Thermogenesis
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DOI：
10.2337/diab.46.5.900
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发表时间：
1997-05
期刊：
影响因子：
7.7
通讯作者：
R. Gimeno;M. Dembski;Xun Weng;N. Deng;A. Shyjan;C. J. Gimeno;F. Iris;S. J. Ellis;E. Woolf;L. Tartaglia
中科院分区：
文献类型：
--
作者：
R. Gimeno;M. Dembski;Xun Weng;N. Deng;A. Shyjan;C. J. Gimeno;F. Iris;S. J. Ellis;E. Woolf;L. Tartaglia
我们已经鉴定出一种新的 cDNA，其编码与哺乳动物棕色脂肪解偶联蛋白 (UCP) 高度同源的蛋白质。与已知的在棕色脂肪组织中特异性表达的 UCP 不同，UCP 同源物 (UCPH) mRNA 在多种组织中表达，主要在人类白色脂肪组织和骨骼肌中表达。在 ob/ob 和 db/db 小鼠的白色脂肪组织中，UCPH 转录物的诱导量是瘦同窝对照小鼠的大约五倍。鼠UCPH在酵母中的表达导致在需要有氧呼吸的条件下生长抑制，但在厌氧条件下不影响生长。此外，通过电位敏感染料 DiOC6 染色可以判断，酵母中 UCPH 的表达会导致线粒体膜电位降低。这些观察结果表明，UCPH 与 UCP 一样，可以解偶联氧化磷酸化。讨论了 UCPH 蛋白是人类生热作用的重要介质的可能性。
We have identified a novel cDNA encoding a protein highly homologous to the mammalian brown fat uncoupling protein (UCP). Unlike the known UCP, which is expressed specifically in brown adipose tissue, the UCP homolog (UCPH) mRNA is expressed in a variety of tissues, with predominant expression in human white adipose tissue and skeletal muscle. In the white adipose tissue of ob/ob and db/db mice, the UCPH transcript is induced approximately fivefold relative to lean littermate controls. Expression of murine UCPH in yeast results in growth inhibition under conditions that require aerobic respiration, but does not affect growth under anaerobic conditions. Furthermore, UCPH expression in yeast causes a decrease in the mitochondrial membrane potential, as judged by staining with the potential-sensitive dye DiOC6. These observations suggest that UCPH, like UCP, uncouples oxidative phosphorylation. The possibility that the UCPH protein is an important mediator of human thermogenesis is discussed.