ELECTRON-PARAMAGNETIC-RES SIGNALS AND ORIENTATION OF CYTOCHROMES IN THE SPINACH CHLOROPLAST THYLAKOID MEMBRANE
ELECTRON-PARAMAGNETIC-RES SIGNALS AND ORIENTATION OF CYTOCHROMES IN THE SPINACH CHLOROPLAST THYLAKOID MEMBRANE
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DOI：
10.1016/0005-2728(82)90060-3
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
VANNGARD, T
中科院分区：
文献类型：
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作者：
BERGSTROM, J;VANNGARD, T
用电子顺磁共振（EPR）技术研究了菠菜叶绿体中的细胞色素。除了先前指定的低自旋血红素信号，细胞色素[Cyt] f（gz 3.51），高电位Cyt B-559（g/z 3.08）和Cyt B-559转化为低电位形式（gz 2.94），高自旋血红素信号由2，3-二氯-5，6-二氰基-1，4-苯醌（DDQ）诱导。该信号不可能是天然形式的Cyt B-563所致。在磁取向的叶绿体中，研究了类囊体膜上Cyt的取向。天然状态和低电位形式的Cyt B-559的血红素平面垂直于膜平面。低温照射氧化的Cyt B-559的取向相同，这表明还原的血红素垂直于膜取向。
The cytochromes in spinach chloroplasts were studied using EPR spectroscopy. In addition to the low-spin heme signals previously assigned, cytochrome [Cyt] f (gz 3.51), high-potential Cyt b-559 (g/z 3.08) and Cyt b-559 converted to a low-potential form (gz 2.94), a high-spin heme signal was induced by 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ). This signal cannot be due to Cyt b-563 in its native form. The orientation of the Cyt in the thylakoid membrane was studied in magnetically oriented chloroplasts. Cyt b-559 in the native state and in the low-potential form has its heme plane perpendicular to the membrane plane. The orientation was the same for Cyt b-559 oxidized by low-temperature illumination, which suggests that the reduced heme is oriented perpendicular to the membrane.