Granulocyte/macrophage origin of glomerular mesangial cells
Granulocyte/macrophage origin of glomerular mesangial cells
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DOI：
10.1532/ijh97.05018
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发表时间：
2005-08-01
影响因子：
2.1
通讯作者：
Ogawa, M
中科院分区：
文献类型：
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作者：
Abe, T;Fleming, PA;Ogawa, M
我们之前通过将单个增强型绿色荧光蛋白（EGFP）阳性HSC衍生的细胞克隆群移植到经致命辐射的小鼠体内，证明了造血干细胞（HSC）产生肾小球系膜细胞的能力。为了更准确地定义系膜细胞衍生的造血分化途径，我们通过移植策略研究了系膜细胞表达与个体造血谱系之间的关系。在一系列克隆 HSC 移植实验中，我们培育了 3 只主要植入粒细胞和巨噬细胞 (GM) 的小鼠，以及 4 只植入 B 细胞或 B 细胞和 T 细胞的小鼠。当对这些小鼠的肾脏进行分析时，表现出高 GM 谱系植入的小鼠显示，与主要淋巴细胞植入的小鼠相比，EGFP 阳性系膜细胞的水平要高得多。对 EGFP 阳性女性供体的男性受者的肾脏进行荧光原位杂交分析，排除了细胞融合是观察到的分化的原因。这些结果支持肾小球系膜细胞与 GM 共享起源的观点。
We previously demonstrated the ability of hematopoietic stem cells (HSCs) to generate glomerular mesangial cells by transplanting clonal populations of cells derived from a single enhanced green fluorescent protein (EGFP)-positive HSC into lethally irradiated mice. To define more precisely the hematopoietic differentiation pathway through which mesangial cells are derived, we studied the relationship between mesangial cell expression and individual hematopoietic lineages by means of a transplantation strategy. In a series of clonal HSC transplantation experiments, we generated 3 mice engrafted predominantly by granulocytes and macrophages (GMs) and 4 mice engrafted with B-cells or with B-cells and T-cells. When the kidneys of these mice were analyzed, the mice exhibiting high GM lineage engraftment revealed much higher levels of EGFP-positive mesangial cells than those with predominantly lymphocyte engraftment. Fluorescence in situ hybridization analysis of the kidneys from a male recipient of an EGFP-positive female donor excluded cell fusion as the cause for the observed differentiation. These results support the notion that glomerular mesangial cells share their origin with GMs.