Effect of leptin on intestinal apolipoprotein AIV in response to lipid feeding
Effect of leptin on intestinal apolipoprotein AIV in response to lipid feeding
复制标题
DOI：
10.1152/ajpregu.2001.281.3.r753
复制
发表时间：
2001-09-01
影响因子：
2.8
通讯作者：
Tso, P
中科院分区：
文献类型：
--
作者：
Doi, T;Liu, M;Tso, P
我们用免疫组化方法测定了静脉注射瘦素后肠上皮细胞中载脂蛋白AIV（apoAIV）的含量。大多数载脂蛋白AIV免疫反应性在未经处理的肠道位于绒毛细胞，而不是隐窝细胞。载脂蛋白AIV免疫染色的区域分布显示十二指肠中载脂蛋白AIV含量较低，空肠中含量较高，空肠向回肠尾部逐渐降低。十二指肠内灌注脂质（4小时）显着增加载脂蛋白AIV免疫反应性在空肠和回肠。同时静脉输注瘦素加十二指肠脂质输注显着抑制载脂蛋白AIV免疫反应。十二指肠脂质输注增加血浆载脂蛋白AIV显着（ELISA法测定），而同时瘦素输注衰减的增加。这些结果表明，瘦素可能通过抑制载脂蛋白AIV在小肠中的合成来调节循环载脂蛋白AIV。
We determined apolipoprotein AIV (apo AIV) content in intestinal epithelial cells using immunohistochemistry when leptin was administered intravenously. Most of the apo AIV immunoreactivity in the untreated intestine was located in the villous cells as opposed to the crypt cells. Regional distribution of apo AIV immunostaining revealed low apo AIV content in the duodenum and high content in the jejunum that gradually decreases caudally toward the ileum. Intraduodenal infusion of lipid (4 h) significantly increased apo AIV immunoreactivity in the jejunum and ileum. Simultaneous intravenous leptin infusion plus duodenal lipid infusion markedly suppressed apo AIV immunoreactivity. Duodenal lipid infusion increased plasma apo AIV significantly (measured by ELISA), whereas simultaneous leptin infusion attenuated the increase. These findings suggest that leptin may regulate circulating apo AIV by suppressing apo AIV synthesis in the small intestine.