Increased levels of glutathione in bronchoalveolar lavage fluid from patients with asthma.
Increased levels of glutathione in bronchoalveolar lavage fluid from patients with asthma.
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DOI：
10.1164/ajrccm/147.6_pt_1.1461
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发表时间：
1993-06
期刊：
影响因子：
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通讯作者：
Lewis J. Smith;Michael Houston;James C. Anderson
中科院分区：
文献类型：
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作者：
Lewis J. Smith;Michael Houston;James C. Anderson
哮喘患者通过支气管肺泡灌洗（BAL）从外周血细胞和细胞中产生大量活性氧（ROS）。ROS产生许多与哮喘相关的病理生理变化，并可能参与其发病机制。虽然抗氧化防御抑制活性氧产生的变化，没有数据可用于局部抗氧化防御哮喘。本研究旨在开始探讨这些防御措施，通过测量超氧化物歧化酶（SOD）和过氧化氢酶的活性和总谷胱甘肽（GSH）水平，从正常受试者和轻度哮喘患者的支气管肺泡灌洗液。对所有受试者进行基线肺功能和乙酰甲胆碱支气管激发试验。通过在三个肺段的每一个中滴注五个20 ml磷酸盐缓冲盐水等分试样来实现BAL。从第一个20 ml等分试样中回收的液体和从接下来的四个等分试样中回收的液体分别标记为支气管液和肺泡液。哮喘患者FEV 1较低（p < 0.005），BAL液回收较少（p < 0.05），支气管嗜酸性粒细胞百分比增加（p < 0.05）。BAL总细胞计数或蛋白浓度无差异。在两组的未浓缩BAL液中均未一致检测到过氧化氢酶活性。SOD活性在支气管和肺泡样本中均被发现，但在两组受试者中相似。哮喘患者支气管液中的GSH浓度较高（23.9 +/- 6.2 vs 13.0 +/- 1.8 microM/mg蛋白; p < 0.05）;肺泡液中的趋势相似（36.5 +/- 9.4 vs 23.3 +/- 3.0 microM/mg蛋白）。（250字处删节）
Patients with asthma generate increased amounts of reactive oxygen species (ROS) from peripheral blood cells and cells recovered by bronchoalveolar lavage (BAL). ROS produce many of the pathophysiologic changes associated with asthma and may contribute to its pathogenesis. Although antioxidant defenses inhibit the changes produced by ROS, no data are available on local antioxidant defenses in asthma. The present study was designed to begin to explore these defenses by measuring superoxide dismutase (SOD) and catalase activities and total glutathione (GSH) levels in BAL fluid from normal subjects and patients with mild asthma. Baseline pulmonary function and methacholine bronchoprovocation tests were performed on all subjects. BAL was achieved by instilling five 20-ml aliquots of phosphate-buffered saline in each of three lung segments. The fluids recovered from the first 20-ml aliquot and that from the next four aliquots were labeled bronchial and alveolar fluid, respectively. Patients with asthma had a lower FEV1 (p < 0.005), less BAL fluid recovered (p < 0.05), and an increased percentage of bronchial eosinophils (p < 0.05). There were no differences in BAL total cell count or protein concentration. Catalase activity was not consistently detected in the unconcentrated BAL fluid from either group. SOD activity was found in both bronchial and alveolar samples, but it was similar in the two groups of subjects. The GSH concentration in bronchial fluid was higher in the patients with asthma (23.9 +/- 6.2 vs 13.0 +/- 1.8 microM/mg protein; p < 0.05); a similar trend was seen in the alveolar fluid (36.5 +/- 9.4 vs 23.3 +/- 3.0 microM/mg protein).(ABSTRACT TRUNCATED AT 250 WORDS)