Optofluidic analysis system for amplification-free, direct detection of Ebola infection.
Optofluidic analysis system for amplification-free, direct detection of Ebola infection.
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DOI：
10.1038/srep14494
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发表时间：
2015-09-25
影响因子：
4.6
通讯作者：
Schmidt H
中科院分区：
文献类型：
--
作者：
Cai H;Parks JW;Wall TA;Stott MA;Stambaugh A;Alfson K;Griffiths A;Mathies RA;Carrion R;Patterson JL;Hawkins AR;Schmidt H
西非高致命性埃博拉出血热的大规模爆发表明，迫切需要能够快速、准确和低复杂性地识别和量化感染的诊断工具。在这里，我们报告的芯片上的样品制备，扩增免费检测和定量的埃博拉病毒的临床样本使用混合光流体集成。在基于PDMS的微流控芯片（自动机）上进行样品制备和目标预浓缩，然后在硅芯片上的液芯光波导中在10分钟内进行单核酸荧光检测。我们表现出良好的特异性，检测限为0.2 pfu/mL和13个数量级的动态范围，远远优于其他扩增的方法。与金标准RT-PCR方法相比，这种芯片级方法和降低的复杂性是便携式仪器的理想选择，可以在护理点提供传染病的即时诊断和持续监测。
The massive outbreak of highly lethal Ebola hemorrhagic fever in West Africa illustrates the urgent need for diagnostic instruments that can identify and quantify infections rapidly, accurately, and with low complexity. Here, we report on-chip sample preparation, amplification-free detection and quantification of Ebola virus on clinical samples using hybrid optofluidic integration. Sample preparation and target preconcentration are implemented on a PDMS-based microfluidic chip (automaton), followed by single nucleic acid fluorescence detection in liquid-core optical waveguides on a silicon chip in under ten minutes. We demonstrate excellent specificity, a limit of detection of 0.2 pfu/mL and a dynamic range of thirteen orders of magnitude, far outperforming other amplification-free methods. This chip-scale approach and reduced complexity compared to gold standard RT-PCR methods is ideal for portable instruments that can provide immediate diagnosis and continued monitoring of infectious diseases at the point-of-care.