Production of lymphocyte-activating factor (Interleukin 1) by macrophages activated with colony-stimulating factors.
Production of lymphocyte-activating factor (Interleukin 1) by macrophages activated with colony-stimulating factors.
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集落刺激因子激活的巨噬细胞产生淋巴细胞激活因子（白细胞介素 1）。
DOI：
10.4049/jimmunol.125.3.1302
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发表时间：
1980
影响因子：
4.4
通讯作者：
R. Shadduck
中科院分区：
文献类型：
--
作者：
R. Moore;J. Oppenheim;J. Farrar;C. Carter;A. Waheed;R. Shadduck
小鼠腹腔渗出液巨噬细胞与 L929 细胞条件培养基一起孵育，刺激其产生淋巴细胞激活因子（LAF、白细胞介素 1）。这种刺激活性被针对部分纯化的L细胞集落刺激因子（CSF）制备的抗血清部分中和，并在凝胶过滤后与骨髓增殖活性共迁移。三种不同的 L 细胞 CSF 制剂（包括一种纯化至均质的制剂）的 LAF 诱导活性取决于 CSF 的浓度。至少需要 1,000 至 3,000 单位的 CSF 活性才能刺激巨噬细胞产生 LAF。刀豆球蛋白 A (Con A) 刺激的脾上清液还含有 CSF 和 LAF 诱导活性，这些活性在凝胶过滤时共同洗脱。与两个 Con A CSF 峰（表观分子量分别为 25,000 和 35,000）共洗脱的 LAF 诱导活性在含有 1,000 至 3,000 单位 CSF 活性（与 L 细胞来源的 CSF 效力相关）的最小稀释度下有效。基于这些数据，提出CSF，无论是L细胞来源还是淋巴来源，不仅具有骨髓增殖活性，而且能够刺激巨噬细胞产生LAF。
Murine peritoneal exudate macrophages incubated with medium conditioned by L929 cells were stimulated to produce lymphocyte-activating factor (LAF, Interleukin 1). This stimulatory activity was partially neutralized by antiserum prepared against partially purified L cell colony-stimulating factor (CSF) and comigrated upon gel filtration with the myeloproliferative activity. LAF-inducing activity of three different L cell CSF preparations, including one purified to homogeneity, was dependent upon the concentration of CSF. A minimum of 1,000 to 3,000 units of CSF activity was required to stimulate macrophagfes to produce LAF. Concanavalin A (Con A) stimulated splenic supernatants also contained CSF and LAF-inducing activities that co-eluted upon gel filtration. LAF-inducing activities co-eluting with the two Con A CSF peaks (apparent m.w. of 25,000 and 35,000) were effective at minimum dilutions containing 1,000 to 3,000 units of CSF activity correlating in potency with L cell-derived CSF. Based on these data, it is proposed that CSF, whether of L cell or lymphoid origin, not only has myeloproliferative activities but also is capable of stimulating macrophages to produce LAF.