Genetic relationships among Arachis species based on AFLP
Genetic relationships among Arachis species based on AFLP
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DOI：
10.1590/s1415-47572002000300017
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发表时间：
2002-01-01
影响因子：
2.1
通讯作者：
Valls, Jose F.M.
中科院分区：
文献类型：
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作者：
Gimenes, Marcos A.;Lopes, Catalina R.;Valls, Jose F.M.
利用扩增片段长度多态性(AFLP)技术对花生属9个组中7个组的20个种进行了遗传关系分析。对9份栽培花生种质资源的遗传多样性水平进行了评价。用三种不同的引物组合对AFLP进行扩增。这些片段在6%变性丙烯酰胺凝胶中分离。共分析了408个片段。平均每个引物组合扩增出135.3条片段，其中Eco RI-Acc/Mse I-CTG的片段数最多，为169条，而Eco RI-ACT/Mse I-CTT的片段数最低，为108条。总体而言，利用AFLP建立的遗传关系与利用形态和可交配性数据建立的分类一致。结果表明，AFLP是建立花生种间亲缘关系的良好标记。用该方法检测到的多态现象高于其他标记，如RAPDS和RFLP。然而，我们的数据表明，仅用三对引物组合的AFLP检测到的多态性仍然太低，不能用于该物种的任何一种遗传研究。
Amplified Fragment Length Polymorphism (AFLP) was used to establish the genetic relationships among 20 species from seven of the nine sections of genus Arachis. The level of polymorphism among nine accessions of the cultivated peanut, A. hypogaea L., was also evaluated. Three combinations of primers were used to amplify the AFLPs. The fragments were separated in 6% denaturing acrylamide gels. A total of 408 fragments were analyzed. An average of 135.3 fragments per primer combination were scored, and the largest number of fragments was 169 using primer combination Eco RI - ACC / Mse I - CTG, while the lowest was 108, with Eco RI - ACT / Mse I - CTT. In general, the genetic relationships established using AFLPs agreed with the classification established using morphology and crossability data. The results indicated that AFLPs are good markers for establishing the relationships among Arachis species. The polymorphism detected in A. hypogaea by this method was higher than the one found with other markers, like RAPDs and RFLPs. However, our data suggest that the polymorphism detected be using AFLP with only three primer combinations is still too low to be used for any kind of genetic study in this species.