Cells with regulatory function of the innate and adaptive immune system in primary Sjogren's syndrome
Cells with regulatory function of the innate and adaptive immune system in primary Sjogren's syndrome
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DOI：
10.1111/j.1365-2249.2009.03966.x
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发表时间：
2009-09-01
影响因子：
4.6
通讯作者：
Zeher, M.
中科院分区：
文献类型：
--
作者：
Szodoray, P.;Papp, G.;Zeher, M.
本研究的目的是描述在原发性干燥综合征(PSS)中具有调节特性的细胞亚群，并将这些细胞群与临床症状相关联。在接受调查的32名患者中，23名患者有腺外表现，9名患者仅有腺体症状。20名健康人作为对照。采用流式细胞仪检测外周血中自然杀伤细胞(NK)、自然杀伤T细胞(NK T)、白细胞介素10(IL)-10产生1型调节性T细胞(TR1)和CD4(+)CD25(+)调节性T细胞(T-reg)的百分率，用双抗体夹心酶联免疫吸附试验(ELISA)检测血清细胞因子IL-4、IL-6、IL-10、肿瘤坏死因子(TNF)-α和干扰素(IFN)-γ的水平。功能测试被用来评估患者和对照组T-reg细胞的抑制特性。PSS患者外周血中NK细胞、NK T细胞和TR1细胞百分率高于对照组，而CD4+CD25(+)T-reg细胞百分率低于对照组。在PSS患者中，与仅有干燥症状的患者相比，EGMS患者的NK T、TR1和CD4(+)CD25(+)T-reg细胞百分比升高。CD4(+)CD25(+)T-reg细胞百分比与唾液酸值呈负相关。体外功能测定显示，与对照组相比，患者的CD4(+)CD25(+)T-reg细胞的抑制活性较低。血清IL-6、TNF-α水平升高，而IL-10水平下降。IL-10水平与TR1细胞百分率呈负相关。PSS中被研究的调节细胞亚群的变化可能有助于疾病的发生和发展。
The aim of the present study was to describe subsets of cells with regulatory properties in primary Sjogren's syndrome (pSS), and to correlate these cell populations with clinical symptoms. Among the 32 investigated patients, 23 had extraglandular manifestations (EGMs), while nine had only glandular symptoms. Twenty healthy individuals served as controls. The percentages of natural killer (NK), natural killer T cells ( NK T), interleukin (IL)-10 producing T regulatory type 1 (Tr1) cells and CD4(+)CD25(+) regulatory T cells (T-reg) cells were determined by flow cytometry and serum cytokine levels of IL-4, IL-6, IL-10, tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma were evaluated by enzyme-linked immunosorbent assay ( ELISA). Functional tests were carried out to assess the suppressor properties of T-reg cells in patients and controls. Peripheral NK, NK T and Tr1 cell percentages were elevated in pSS, while CD4(+)CD25(+) T-reg cells showed reduced frequencies in patients compared to controls. In pSS, elevated percentages of NK T, Tr1 and CD4(+)CD25(+) T-reg cells were observed in patients with EGMs, when compared to patients with sicca symptoms only. CD4(+)CD25(+) T-reg cell percentages showed a negative correlation with sialometry values. The in vitro functional assay demonstrated lower suppression activity of CD4(+)CD25(+) T-reg cells in patients compared to controls. Serum IL-6 and TNF-alpha levels were elevated, while IL-10 was decreased in patients compared to controls. Negative correlation was found between IL-10 levels and the percentages of Tr1 cells. Changes in the investigated subsets of regulatory cells in pSS may contribute to the development and progression of the disease.