Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov., obligately anaerobic bacteria from the human oral cavity, and emended description of the genus Oribacterium.
Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov., obligately anaerobic bacteria from the human oral cavity, and emended description of the genus Oribacterium.
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DOI：
10.1099/ijs.0.060988-0
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发表时间：
2014-08
影响因子：
2.8
通讯作者：
M. Sizova;Paul A. Muller;David Stancyk;N. Panikov;M. Mandalakis;Amanda Hazen;Tine Hohmann;Sebastian N. Doerfert;W. Fowle;A. Earl;K. Nelson;S. Epstein
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文献类型：
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作者：
M. Sizova;Paul A. Muller;David Stancyk;N. Panikov;M. Mandalakis;Amanda Hazen;Tine Hohmann;Sebastian N. Doerfert;W. Fowle;A. Earl;K. Nelson;S. Epstein
从人龈下牙菌斑中分离到3株严格厌氧、革兰氏阳性、非孢子形成、棒状、活动菌，分别命名为ACB1(T)、ACB7(T)和ACB8。所有菌株的生长都需要酵母提取物。菌株ACB1(T)和ACB8能在葡萄糖、乳糖、麦芽糖、麦芽糖糊精和棉子糖上生长；菌株ACB7(T)仅在蔗糖上生长较弱。生长温度范围为30 ~ 42℃，最适生长温度为37℃。菌株ACB1(T)主要代谢发酵终产物为乙酸和乳酸；菌株ACB7(T)和ACB8的唯一产物是乙酸酯。菌株ACB1(T)的主要脂肪酸为C（14:0）、C（16:0）、C(16:1)ω7c二甲基醛（DMA）和C(18:1)ω7c DMA。菌株ACB7(T)的主要脂肪酸为C（12:0）、C（14:0）、C（16:0）、C(16:1)ω7c和C(16:1)ω7c DMA。肽聚糖的水解产物中含有中二氨基戊酸，表明肽聚糖为A1γ型。不同菌株基因组DNA G+C含量在42 ~ 43.3%之间。根据16S rRNA基因序列系统发育，菌株ACB1(T)、ACB8和ACB7(T)在Oribacterium属内形成了两个独立的分支，序列相似性为98.1 ~ 98.6%。预测菌株ACB1(T)、ACB8、ACB7(T)与O. sinus F0268之间的DNA-DNA杂交值<70%。基于不同的基因型和表型特征，菌株ACB1(T)和ACB8以及菌株ACB7(T)被认为代表了两个不同的Oribacterium属物种，并提出了Oribacterium parvum sp. 11和Oribacterium asaccharolyticum sp. 11的名称。类型菌株分别为ACB1(T) (= DSM 24637(T) = HM-481（T) = ATCC BAA-2638(T)）和ACB7(T) (= DSM 24638(T) = HM-482（T) = ATCC BAA-2639(T)）。
Three strictly anaerobic, Gram-positive, non-spore-forming, rod-shaped, motile bacteria, designated strains ACB1(T), ACB7(T) and ACB8, were isolated from human subgingival dental plaque. All strains required yeast extract for growth. Strains ACB1(T) and ACB8 were able to grow on glucose, lactose, maltose, maltodextrin and raffinose; strain ACB7(T) grew weakly on sucrose only. The growth temperature range was 30-42 °C with optimum growth at 37 °C. Major metabolic fermentation end products of strain ACB1(T) were acetate and lactate; the only product of strains ACB7(T) and ACB8 was acetate. Major fatty acids of strain ACB1(T) were C(14 : 0), C(16 : 0), C(16 : 1)ω7c dimethyl aldehyde (DMA) and C(18 : 1)ω7c DMA. Major fatty acids of strain ACB7(T) were C(12 : 0), C(14 : 0), C(16 : 0), C(16 : 1)ω7c and C(16 : 1)ω7c DMA. The hydrolysate of the peptidoglycan contained meso-diaminopimelic acid, indicating peptidoglycan type A1γ. Genomic DNA G+C content varied from 42 to 43.3% between strains. According to 16S rRNA gene sequence phylogeny, strains ACB1(T), ACB8 and ACB7(T) formed two separate branches within the genus Oribacterium, with 98.1-98.6% sequence similarity to the type strain of the type species, Oribacterium sinus. Predicted DNA-DNA hybridization values between strains ACB1(T), ACB8, ACB7(T) and O. sinus F0268 were <70%. Based on distinct genotypic and phenotypic characteristics, strains ACB1(T) and ACB8, and strain ACB7(T) are considered to represent two distinct species of the genus Oribacterium, for which the names Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov. are proposed. The type strains are ACB1(T) ( = DSM 24637(T) = HM-481(T) = ATCC BAA-2638(T)) and ACB7(T) ( = DSM 24638(T) = HM-482(T) = ATCC BAA-2639(T)), respectively.