Mucin (MUC) gene expression in human pancreatic adenocarcinoma and chronic pancreatitis: a potential role of MUC4 as a tumor marker of diagnostic significance.
Mucin (MUC) gene expression in human pancreatic adenocarcinoma and chronic pancreatitis: a potential role of MUC4 as a tumor marker of diagnostic significance.
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发表时间：
2001-12
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通讯作者：
M. Andrianifahanana;N. Moniaux;B. Schmied;J. Ringel;H. Friess;M. Hollingsworth;M. Büchler;J. Aubert;S. Batra
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作者：
M. Andrianifahanana;N. Moniaux;B. Schmied;J. Ringel;H. Friess;M. Hollingsworth;M. Büchler;J. Aubert;S. Batra
粘蛋白是一种重要的生物分子，在病理条件下经常表现出表达改变。为了寻找一种独特而可靠的胰腺腺癌特异性标志物，我们研究了不同MUC基因在胰腺肿瘤和肿瘤细胞系、慢性胰腺炎和正常胰腺中的表达。实验设计采用MUC1、MUC2、MUC3、MUC4、MUC5AC、MUC5B、MUC6和MUC7基因特异性引物逆转录pcr和RNA槽印迹分析了16例胰腺肿瘤组织、10例慢性胰腺炎组织、7例正常胰腺组织和15例胰腺肿瘤细胞系的总RNA。结果：我们的研究结果显示，在所有检测的粘蛋白中，只有MUC4表现出胰腺腺癌特异性的差异表达。事实上，大量的肿瘤组织样本（16个中的12个）和肿瘤细胞系（15个中的11个）表达MUC4 mRNA，而来自慢性胰腺炎（10个中的0个）和正常胰腺（7个中的0个）组织的样本没有表现出任何可检测到的MUC4 mRNA水平。相反，与正常胰腺样品相比，其他黏蛋白的表达没有明显变化。总之，本研究表明胰腺黏液蛋白MUC4是一种肿瘤相关的黏液蛋白。此外，本研究还介绍了一种区分胰腺腺癌和胰腺炎的新方法。未来对MUC4在胰腺腺癌中所起作用的研究可能有助于制定这种恶性肿瘤的诊断和治疗策略。
PURPOSE Mucins are important biomolecules that frequently display an altered expression under pathological conditions. In a search for a unique and reliable marker(s) specific for pancreatic adenocarcinoma, we investigated the expression of different MUC genes in pancreatic tumors and tumor cell lines, in chronic pancreatitis, and in the normal pancreas. EXPERIMENTAL DESIGN Total RNA from 16 pancreatic tumors, 10 chronic pancreatitis tissues, 7 normal pancreas tissues, and 15 pancreatic tumor cell lines were analyzed by reverse transcription-PCR with primers specific for MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC5B, MUC6, and MUC7 genes and by RNA slot blot analyses. RESULTS Our results revealed that of all of the mucins examined, only MUC4 displayed a differential expression that was specific for pancreatic adenocarcinoma. Indeed, a substantial number of tumor tissue samples (12 of 16) and tumor cell lines (11 of 15) expressed MUC4 mRNA, whereas samples from chronic pancreatitis (0 of 10) and the normal pancreas (0 of 7) tissues failed to exhibit any detectable level of this mucin. In contrast, no significant alteration was observed in the expression of the other mucins relative to that in the normal pancreas samples. CONCLUSIONS Overall, this work demonstrates that pancreatic mucin MUC4 is a tumor-associated mucin. Furthermore, the present study introduces a novel avenue to discriminate between pancreatic adenocarcinoma and pancreatitis. Future investigations of the role played by MUC4 in pancreatic adenocarcinoma may prove to be useful in the formulation of strategies for the diagnosis and therapeutic treatment of this malignancy.