Effect of CO2 removal on hydrogen production by fermentation

Effect of CO2 removal on hydrogen production by fermentation
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DOI:
10.1016/s0360-3199(97)00117-1
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发表时间:
1998-07-01
影响因子:
7.2
通讯作者:
Kadokura, N
Kadokura, N
中科院分区:
工程技术2区
文献类型:
--
作者:
Tanisho, S;Kuromoto, M;Kadokura, N

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从两个角度研究了使用糖蜜作为底物的发酵生产中氢产量的提高;即首先通过生产途径的控制,其次通过生长的营养源条件的控制。结果表明,产气肠杆菌菌株E.82005的产物之一琥珀酸是通过积累CO2诱导产生的,与乙酸、乙醇和其他产物同时产生。当Ar或H-2吹入培养液中时,作为H-2进化基础物质的残留NADH的量增加。从这些结果可以得出结论,从培养液中去除CO2可以促进氢气产率的提高。还表明,细菌生长所需的充足氮源的存在是获得全部氢气产量的必要条件。结合去除 CO2 和提供充足氮源的实验发现,以葡萄糖计,1 mol 糖可产生 1.58 mol 氢。 (C) 1998 年国际氢能协会。
The improvement of the yield of hydrogen at fermentative production using molasses as a substrate was investigated from two points of view; i.e. firstly, through the control of production pathway and secondly, by the condition of nutrient source for growing. It was shown that succinate, one of the products of Enterobacter aerogenes strain E.82005, was produced inductively by accumulation of CO2 which was produced simultaneously with acetate, ethanol and other products. The amount of residual NADH which is the base material of H-2 evolution increased when Ar or H-2 was blown into the culture liquid. From these results, it was concluded that the removal of CO2 from culture liquid effected the promotion of the yield of hydrogen. It was also shown that the existence of sufficient nitrogen source for bacterial growth was a necessary condition to get entire yield of hydrogen. The combination of experiments to remove CO2 and to give sufficient nitrogen source was found to yield 1.58 mol hydrogen from 1 mol sugar in terms of glucose. (C) 1998 International Association for Hydrogen Energy.