Ethylene Induced Flower Opening, Expression of Receptor Genes, and Antioxidant Defensive Responses in Cut Roses
Ethylene Induced Flower Opening, Expression of Receptor Genes, and Antioxidant Defensive Responses in Cut Roses
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DOI：
10.1134/s1021443720040081
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发表时间：
2020-07
影响因子：
1.4
通讯作者：
F. Khatami;F. Najafi;F. Yari;R. Khavari-nejad;K. Takaki;T. Okumura;K. Takahashi
中科院分区：
文献类型：
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作者：
F. Khatami;F. Najafi;F. Yari;R. Khavari-nejad;K. Takaki;T. Okumura;K. Takahashi
摘要本研究旨在探讨乙烯对月季切花生理和分子响应的影响。在相同的瓶插室条件下，在部分开放期，用10 ppm外源乙烯处理两个乙烯敏感性不同的切花玫瑰品种（“Amada+”和“Brillante”）24 h。然后通过气相色谱、UV/维斯光谱和qRT-PCR在部分和完全开放阶段检查品种。高敏感性品种的总蛋白含量较低，乙烯释放量和抗氧化活性较高。无论是外源施加乙烯或内源产生的一个导致抗氧化活性增加，总蛋白质含量下降。这一观察结果可能是因为乙烯诱导的花朵开放和衰老增强了自由基的产生和蛋白酶的激活。qRT-PCR分析表明，高敏感品种在商品期乙烯信号转导基因（RhETR 1，3和RhCTR 1，2）的表达量显著增加。而低感品种在全开放期RhETR 1，3基因表达显著上调。乙烯诱导的反应高度依赖于品种的乙烯合成和感知能力。
AbstractThe present research aimed to investigate the effect of ethylene on the physiological and molecular responses of the cut roses,Rosa hybridaL. Two cut rose cultivars (‘Amada +’ and ‘Brillante’) with different ethylene sensitivity were treated by 10 ppm exogenous ethylene for 24 h under identical vase life room conditions at partially open stage. The cultivars were then examined at both partially and fully open stage by gas chromatography, UV/Vis spectroscopy, and qRT-PCR. The cultivar with high sensitivity had lower total protein contents and showed higher ethylene production and antioxidant activity. Either exogenously applied ethylene or endogenously produced one resulted in increased antioxidant activity and decreased total protein contents. This observation is possibly because of enhanced free radical generation and protease activation with ethylene-induced flower opening and senescence. The qRT-PCR analysis showed a significant increase in the expression of ethylene signal transduction genes (RhETR1,3andRhCTR1,2) of the treated cultivars with high sensitivity at commercial stage. However, there was a significant up-regulation of theRhETR1,3genes in the cultivars with low sensitivity at fully-open stage. The ethylene induced responses were found highly dependent on the cultivar ability in both ethylene synthesis and perception.