Correlations of oral bacterial arginine and urea catabolism with caries experience.
Correlations of oral bacterial arginine and urea catabolism with caries experience.
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DOI：
10.1111/j.1399-302x.2008.00477.x
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发表时间：
2009-04
影响因子：
--
通讯作者：
Burne RA
中科院分区：
文献类型：
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作者：
Nascimento MM;Gordan VV;Garvan CW;Browngardt CM;Burne RA
口腔细菌产碱在菌斑pH动态平衡中起着关键作用，可能是龋病发生的主要障碍。为了确定口腔样本从精氨酸或尿素产生氨的能力是否与龋齿经历有关，对45名成人受试者的唾液和牙菌斑样本的精氨酸脱亚胺酶系统(ADS)和尿素酶活性进行了测定。受试者根据龋齿状况分为三组：13名无龋者(CF)(龋齿、缺牙和充填牙=0)；21名龋病活跃者(CA)(龋齿≥4)；11名有龋齿(CE)者(龋齿=0；缺失和充填牙>；0)。实时聚合酶链式反应被用来量化样品中某些产酸或产碱微生物的比例。在所有组中，菌斑样本从测试底物产生的氨量普遍高于唾液样本产生的氨量。CF组唾液ADS活性和斑块尿素酶活性均显著高于CA组(P=0.0004和P=0.014)。CA组唾液和牙菌斑中变形链球菌的比例显著高于CF组(P=0.0153和P=0.0009)。CA组尿素酶活性与变形链球菌水平呈负相关(P<；0.0001)。这项研究支持了这样的理论，即龋病风险的增加与口腔定植细菌产碱能力的降低有关；为进一步了解口腔产碱在健康和疾病中的作用提供了令人信服的证据。
Alkali generation by oral bacteria plays a key role in plaque pH homeostasis and may be a major impediment to the development of dental caries. To determine if the capacity of oral samples to produce ammonia from arginine or urea was related to caries experience, the arginine deiminase system (ADS) and urease activity in saliva and dental plaque samples were measured in 45 adult subjects. The subjects were divided into three groups according to caries status; 13 caries-free (CF) individuals (decayed, missing, and filled teeth = 0); 21 caries-active (CA) individuals (decayed teeth ≥ 4); and 11 caries-experienced (CE) individuals (decayed teeth = 0; missing and filled teeth > 0). Real-time polymerase chain reaction was used to quantify the proportion of certain acid- or alkali-producing organisms in the samples. The amount of ammonia generated from the test substrates by plaque samples was generally higher than that produced by salivary samples in all groups. Significantly higher levels of salivary ADS activity and plaque urease activity were observed in CF subjects compared to CA subjects (P = 0.0004 and P = 0.014, respectively). The proportions of Streptococcus mutans from saliva and dental plaque of CA subjects were significantly higher than those from the CF group (P = 0.0153 and P = 0.0009, respectively). In the CA group, there was an inverse relationship between urease activity and the levels of S. mutans (P < 0.0001). This study supports the theory that increased caries risk is associated with reduced alkali-generating capacity of the bacteria colonizing the oral cavity; providing compelling evidence to further our understanding of oral alkali-generation in health and disease.