Analysis of gene-expression profiles by oligonucleotide microarray in children with influenza
Analysis of gene-expression profiles by oligonucleotide microarray in children with influenza
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DOI：
10.1099/vir.0.81670-0
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发表时间：
2006-06-01
影响因子：
3.8
通讯作者：
Morishima, Tsuneo
中科院分区：
文献类型：
--
作者：
Kawada, Jun-ichi;Kimura, Hiroshi;Morishima, Tsuneo
为了阐明宿主对流感病毒应答的机制，采用寡核苷酸芯片技术研究了流感患儿外周血基因表达谱。在流感急性期，200个基因表达上调，20个基因表达下调。干扰素调节基因，如干扰素诱导蛋白与tetratricopeptide重复2（IFIT2）和vipirin，强烈上调，在急性期。基因本体分析表明，免疫反应基因是高度过度表达的上调基因。同时还研究了伴有和不伴有热性惊厥的流感患者的基因表达谱。与无热性惊厥患者相比，热性惊厥患者中有22个基因表达上调，5个基因表达下调。这些结果有助于阐明流感及其神经系统并发症的发病机制。
In order to clarify the mechanism of the host response to influenza virus, gene-expression profiles of peripheral blood obtained from paediatric patients with influenza were investigated by oligonucleotide microarray. In the acute phase of influenza, 200 genes were upregulated and 20 genes were downregulated compared with their expression in the convalescent phase. Interferon-regulated genes, such as interferon-induced protein with tetratricopeptide repeats 2 (IFIT2) and vipirin, were strongly upregulated in the acute phase. Gene ontology analysis showed that immune response genes were highly overrepresented among the upregulated genes. Gene-expression profiles of influenza patients with and without febrile convulsion were also studied. In patients with febrile convulsion, 22 genes were upregulated and five were downregulated compared with their expression in patients without febrile convulsion. These results should help to clarify the pathogenesis of influenza and its neurological complications.