Regulation of miRNA abundance by RNA binding protein TOUGH in Arabidopsis
Regulation of miRNA abundance by RNA binding protein TOUGH in Arabidopsis
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DOI：
10.1073/pnas.1204915109
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发表时间：
2012-07-31
影响因子：
11.1
通讯作者：
Yu, Bin
中科院分区：
文献类型：
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作者：
Ren, Guodong;Xie, Meng;Yu, Bin
microRNA（miRNAs）是植物和动物基因表达的调节因子。miRNAs的生物发生受到精确控制，以确保生物体的正常发育。在这里，我们报告说，坚韧（TGH）是DCL 1-HYL 1-SERRATE复合物的一个组成部分，该复合物处理miRNA的初级转录物[即，primarymiRNAs（pri-miRNAs）]转化为miRNAs。缺乏TGH会损害多种体外DCL活性，并减少体内miRNA和siRNA的积累。TGH是一种RNA结合蛋白，在体内结合pri-miRNAs和前体miRNAs，并有助于pri-miRNA-HYL 1相互作用。这些结果表明，TGH可能通过促进DCL 1切割效率和/或pri-miRNAs的募集来调节miRNAs的丰度。
MicroRNAs (miRNAs) are regulators of gene expression in plants and animals. The biogenesis of miRNAs is precisely controlled to secure normal development of organisms. Here we report that TOUGH (TGH) is a component of the DCL1-HYL1-SERRATE complex that processes primary transcripts of miRNAs [i.e., primary miRNAs (pri-miRNAs)] into miRNAs in Arabidopsis. Lack of TGH impairs multiple DCL activities in vitro and reduces the accumulation of miRNAs and siRNAs in vivo. TGH is an RNA-binding protein, binds pri-miRNAs and precursor miRNAs in vivo, and contributes to pri-miRNA-HYL1 interaction. These results indicate that TGH might regulate abundance of miRNAs through promoting DCL1 cleavage efficiency and/or recruitment of pri-miRNAs.