MELANIN ENDOCYTOSIS BY CULTURED MAMMALIAN-CELLS - A MODEL FOR MELANIN IN A CELLULAR ENVIRONMENT
MELANIN ENDOCYTOSIS BY CULTURED MAMMALIAN-CELLS - A MODEL FOR MELANIN IN A CELLULAR ENVIRONMENT
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DOI：
10.1016/0014-4827(83)90362-2
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发表时间：
1983-01-01
影响因子：
3.7
通讯作者：
HATCHELL, MC
中科院分区：
文献类型：
--
作者：
PAJAK, S;HOPWOOD, LE;HATCHELL, MC
报道了从牛眼睛中提取的天然真黑色素和合成多巴黑色素在中国仓鼠卵巢（CHO）细胞中的掺入。这一过程至少持续8小时，呈线性。电镜显示黑色素以单个颗粒或成组颗粒的形式被吞噬，进入细胞溶酶体，随后颗粒被降解。摄取和降解过程的一般特征类似于黑素体并入角质形成细胞的过程。摄入黑色素的CHO细胞总体上显示出与无色素的CHO细胞非常相似的特性：中等浓度的色素对细胞分裂、氧气消耗和镀效率没有太大的影响。这表明CHO细胞系统可能对细胞环境中色素的研究有用；无色素的CHO细胞具有很好的特征，可以作为很好的对照。初步应用报告：证明金属离子（Al3+）结合到CHO细胞使用黑色素作为载体；以及黑色素在细胞光照射过程中提高氧气消耗速率的能力。
The incorporation of natural eumelanin from bovine eyes and synthetic, dopa melanin into Chinese hamster ovary (CHO) cells is reported. The process is linear for at least 8 h. EM showed phagocytosis of melanin, either as a single granule or in groups of granules, into cell lysosomes with subsequent degradation of the granule. The general features of the ingestion and degradation processes mimic those of the incorporation of melanosomes into keratinocytes. CHO cells with ingested melanin in general revealed properties very similar to those of the pigment-free CHO cell:cell division, O2 consumption and plating efficiency were not greatly altered by moderate concentrations of pigment. This suggests that the CHO cell system may be useful for the study of pigment in a cellular environment; pigment-free CHO cells are well characterized and can serve as a good control. Preliminary applications are reported: demonstrations of incorporation of metal ions (Al3+) into CHO cells using melanin as a carrier; and the ability of melanin to enhance the rate of O2 consumption during photoirradiation of the cells.