Functional characterization of Kid-Kis and MazF-MazE in Sf9 cells and Mythimna separata embryos
Functional characterization of Kid-Kis and MazF-MazE in Sf9 cells and Mythimna separata embryos
复制标题
Sf9 细胞和粘虫胚胎中 Kid-Kis 和 MazF-MazE 的功能表征
DOI：
10.1016/j.pestbp.2021.104814
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发表时间：
2021
影响因子：
4.7
通讯作者：
Zhihui Zhu
中科院分区：
文献类型：
--
作者：
Qiuyuan Zhang;Yicheng Zhou;Yunfei Li;Bahar Ali;Zhihui Zhu
毒素-抗毒素（TA）系统由毒素及其解毒剂抗毒素组成，广泛存在于细菌和真核生物系统中。然而，没有关于TA系统的工作已在昆虫中报道。我们对草地贪夜蛾细胞和粘虫胚胎中的Kid-Kis和MazF-MazE TA系统进行了表征，并观察到Kid和MazF毒素具有很高的毒性。Kid质粒转染的Sf 9细胞凋亡率为24.37%，MazF单独转染的Sf 9细胞凋亡率为29.47%。而其同源抗毒素毒性有限。两种毒素均可诱导细胞凋亡和坏死。Kis和迷宫抗毒素都以剂量依赖性方式部分中和毒性，迷宫在1：4的毒素：抗毒素比例下几乎完全中和，细胞存活率分别为81%和97%。我们的研究结果表明，MazF-MazE是一个很好的候选模块，在昆虫中的应用，如在开发新的不育昆虫技术（SIT）。
Toxin-antitoxin (TA) systems are comprised of a toxin and its antidote antitoxin and are widely present in.bacterial and in eukaryotic systems. However, no work regarding TA systems has been reported in insects. We.characterized the Kid-Kis and MazF-MazE TA systems in Spodoptera frugiperda cells and Mythimna separata embryos.and observed that the Kid and MazF toxins were highly toxic. In Sf9 cells transfected with Kid plasmid and.MazF alone, the apoptosis rate was 24.37% and 29.47%, respectively. Whereas the toxicity of their cognate.antitoxins were limited. Both apoptosis and necrosis were induced by the two toxins. Both the Kis and MazE.antitoxins partly neutralized toxicity in a dose-dependent manner, with MazE accomplishing almost full.neutralization at a 1:4 toxin:antitoxin ratio, the cell survival rate was 81% and 97%, respectively. Our results.indicate that MazF-MazE is a good candidate module for application in insects, such as in developing new sterile.insect technique (SIT).