The Loop 2 Region of Ribosomal Protein uS5 Influences Spectinomycin Sensitivity, Translational Fidelity, and Ribosome Biogenesis
The Loop 2 Region of Ribosomal Protein uS5 Influences Spectinomycin Sensitivity, Translational Fidelity, and Ribosome Biogenesis
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DOI：
10.1128/aac.01186-16
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发表时间：
2017-02-01
影响因子：
4.9
通讯作者：
O'Connor, Michael
中科院分区：
文献类型：
--
作者：
Kamath, Divya;Gregory, Steven T.;O'Connor, Michael
核糖体蛋白 uS5 是核糖体小亚基的重要组成部分，参与亚基组装、翻译保真度的维持以及核糖体对抗生素大观霉素的反应。虽然影响解码的许多特征性 uS5 突变映射到其与 uS4 的界面，但最近的工作表明，远离 uS4-uS5 界面的残基也可以影响解码过程。我们针对这样一个界面远程区域，即环 2 区域（残基 20 至 31），在埃希氏菌中进行诱变。大肠杆菌并产生了 21 个独特的突变体。大多数环 2 改变会导致对壮观霉素的抗性并影响翻译的保真度。然而，只有少数表现出 rRNA 加工改变或核糖体生物合成缺陷。
Ribosomal protein uS5 is an essential component of the small ribosomal subunit that is involved in subunit assembly, maintenance of translational fidelity, and the ribosome's response to the antibiotic spectinomycin. While many of the characterized uS5 mutations that affect decoding map to its interface with uS4, more recent work has shown that residues distant from the uS4-uS5 interface can also affect the decoding process. We targeted one such interface-remote area, the loop 2 region (residues 20 to 31), for mutagenesis in Escherichia. coli and generated 21 unique mutants. A majority of the loop 2 alterations confer resistance to spectinomycin and affect the fidelity of translation. However, only a minority show altered rRNA processing or ribosome biogenesis defects.