FOURIER-TRANSFORM INFRARED STUDY ON THE PHASE-TRANSITIONS OF A 1,2-BIS(MYRISTOYLAMIDO)-1,2-DEOXYPHOSPHATIDYLCHOLINE WATER-SYSTEM
FOURIER-TRANSFORM INFRARED STUDY ON THE PHASE-TRANSITIONS OF A 1,2-BIS(MYRISTOYLAMIDO)-1,2-DEOXYPHOSPHATIDYLCHOLINE WATER-SYSTEM
复制标题
DOI：
10.1021/la00080a034
复制
发表时间：
1988-03-01
期刊：
影响因子：
3.9
通讯作者：
SUNAMOTO, J
中科院分区：
文献类型：
--
作者：
KAWAI, T;UMEMURA, J;SUNAMOTO, J
对 44 g% l, 2-双（肉豆蔻酰氨基）-l, 2-脱氧磷脂酰胆碱（DDPQ-56 g% 水系统）进行了热致相变的傅里叶变换红外研究。为了监测温度引起的结构变化，从差异光谱计算了参数 AA/AT，它是光谱特征总变化的敏感量度。这里是吸光度的峰谷差异差异光谱中特定谱带的标度和AT是相应的温度差，AA/AT~T曲线显示对应于反对称CH2拉伸(2920cm'1)和酰胺II(1558cm'1)谱带的相变I(在16Q和II(在22℃))的两个最大值。对于反对称P02拉伸(1214cm'1)和CN拉伸(970cm'1)谱带，出现三个。 AA/AT~T曲线中的最大值对应于相变I、II和III（28°C）。这些事实表明，在相变I和II时，疏水性亚甲基链和酰胺基团的行为与亲水性磷酸基和胆碱基团不同，几乎所有DDPC分子部分都发生结构变化，但在相变III时，主要发生磷酸基和胆碱基团的结构变化。
Fourier transform infrared studies on the thermotropic phase transitions have been made of a 44 g% l, 2-bis (myristoylamido)-l, 2-deoxyphosphatidylcholine (DDPQ-56 g% water system. In orderto monitor the temperature-induced structrual change, a parameter AA/AT, which is a sensitive measure of the total change in thespectral feature, was calculated from difference spectra. Here is the peak to valley difference in theabsorbance scale of a particular band in the difference spectra and AT is the corresponding temperature difference. The AA/AT~ T curves show two maxima corresponding to the phase transitions I (at 16 Q and II (at 22 C) for the antisymmetric CH2 stretching (2920 cm'1) and amide II (1558 cm" 1) bands. For the antisymmetric P02 stretching (1214 cm'1) and the CN stretching (970 cm'1) bands, there appear three maxima in the AA/AT~ T curve corresponding to the phase transitions I, II, and III (at 28 C). These facts suggest that the hydrophobic methylene chains and amide groups behave in a different manner from the hydrophilic phosphate and choline groups on heating. At the phase transitions I and II, almost all parts of the DDPC molecule are subjected to the structural change. At the transition III, however, the structural change of the phosphateand choline groups mainly occurs.