Evaluation of a rapid real-time RT-PCR assay for detection of enterovirus RNA in cerebrospinal fluid specimens
Evaluation of a rapid real-time RT-PCR assay for detection of enterovirus RNA in cerebrospinal fluid specimens
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DOI：
10.1016/s1386-6532(02)00032-x
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发表时间：
2002-07-01
影响因子：
8.8
通讯作者：
Mertens, AH
中科院分区：
文献类型：
--
作者：
Verstrepen, WA;Bruynseels, P;Mertens, AH
背景：我们之前描述了基于荧光探针和引物的单管实时肠病毒逆转录酶聚合酶链反应（RT-PCR）检测的特点，该检测指向肠病毒基因组5'-非翻译区（UTR）的高度保守序列。目的：评价该方法对疑似病毒性脑膜炎患者大量脑脊液标本的检测效果。研究设计：对2000年3月至2001年11月收到的脑脊液标本进行实时肠道病毒RT-PCR和病毒培养。回顾性回顾患者记录以获得最终临床诊断。结果：186份脑脊液标本中，31例肠病毒培养阳性，45例实时RT-PCR检测到肠病毒RNA。实时RT-PCR对病毒性脑膜炎临床诊断的敏感性为72.6%，而病毒培养的敏感性仅为57.4%。在一些白细胞计数低的标本中也发现了肠病毒RNA。结论：我们证实实时肠道病毒RT-PCR检测脑脊液标本的敏感性明显高于病毒培养。(C) 2002 Elsevier Science B.V.版权所有
Background: We previously described the characteristics of a single-tube real-time enterovirus reverse transcriptase polymerase chain reaction (RT-PCR) assay based on a fluorogenic probe and primers directed to highly conserved sequences in the 5'-untranslated region (UTR) of the enterovirus genome. Objectives: To evaluate the performance of the assay on a larger number of cerebrospinal fluid (CSF) specimens from patients suspected of having viral meningitis. Study design: Real-time enterovirus RT-PCR and viral culture were performed on CSF specimens received from March 2000 to November 2001. Patient records were retrospectively reviewed for final clinical diagnosis. Results: From the 186 CSF specimens tested, culture was positive for enterovirus in 31 cases, whereas real-time RT-PCR detected enterovirus RNA in 45 CSF specimens. The sensitivity of real-time RT-PCR in relation to the clinical diagnosis of viral meningitis was 72.6%, whereas the sensitivity of viral culture reached only 57.4%. Enterovirus RNA was also found in a number of specimens with low leukocyte counts. Conclusions: We confirm that the real-time enterovirus RT-PCR assay for CSF specimens is significantly more sensitive than viral culture. (C) 2002 Elsevier Science B.V. All rights reserved.