Cooperative Adsorption of Lipoprotein Phospholipids, Triglycerides, and Cholesteryl Esters Are a Key Factor in Nonspecific Adsorption from Blood Plasma to Antifouling Polymer Surfaces
Cooperative Adsorption of Lipoprotein Phospholipids, Triglycerides, and Cholesteryl Esters Are a Key Factor in Nonspecific Adsorption from Blood Plasma to Antifouling Polymer Surfaces
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DOI：
10.1021/ja402126t
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发表时间：
2013-05-08
影响因子：
15
通讯作者：
Huck, Wilhelm T. S.
中科院分区：
文献类型：
--
作者：
Gunkel, Gesine;Huck, Wilhelm T. S.
非特异性蛋白质吸附是在生物介质中使用聚合物材料的核心挑战。虽然吸附的蛋白质的量可以降低，但当暴露于未稀释的血清或血浆时，很少有表面是蛋白质抗性的。这种结垢和吸附蛋白质的基本原理仍有待确定。在这里，我们研究了从未稀释的人血浆吸附到三种不同的聚合物刷。我们的研究表明，聚合物结构不影响蛋白质吸附。此外，我们确定了98个血浆蛋白，仍然污垢电流“蛋白质抗性”聚合物刷。对主要吸附蛋白质的详细研究揭示了脂蛋白和低密度脂蛋白在血浆污染聚合物生物材料中的核心作用。然而，虽然载脂蛋白B100被发现作为一个主要的污垢蛋白在我们的质谱筛选，对脂蛋白的各个组成部分的研究表明，它不是载脂蛋白B100，但磷脂，甘油三酯和胆固醇酯的混合物，在脂蛋白吸附中发挥了重要作用。
Nonspecific protein adsorption is a central challenge for the use of polymeric materials in biological media. While the quantity of adsorbed protein can be lowered, very few surfaces are protein resistant when exposed to undiluted serum or plasma. The underlying principles of this fouling and the adsorbing proteins remain to be identified. Here, we investigated adsorption from undiluted human blood plasma to three different polymer brushes. Our study showed that the polymer structure does not influence which proteins adsorb. Further, we identified 98 plasma proteins that still foul current "protein-resistant" polymer brushes. Detailed studies into the major adsorbing protein revealed the central role that lipoproteins and low density lipoprotein in particular play in fouling of plasma to polymeric biomaterials. However, although apolipoprotein B100 is found as a major fouling protein in our mass spectrometry screening, studies on individual components of lipoproteins show that it is not apoB100 but a mixture of phospholipids, triglycerides, and cholesteryl esters that plays a major role in lipoprotein adsorption.