Elucidation of the HIV‐1 virucidal mechanism of methylene blue photosensitization and the effect on primary isolates
Elucidation of the HIV‐1 virucidal mechanism of methylene blue photosensitization and the effect on primary isolates
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阐明亚甲蓝光敏作用的 HIV-1 杀病毒机制及其对原代分离株的影响
DOI：
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发表时间：
2000
影响因子：
12.7
通讯作者：
K. Ikebuchi
中科院分区：
文献类型：
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作者：
Takashi Owada;Yoshiko Yamada;H. Abe;J. Hirayama;H. Ikeda;S. Sekiguchi;K. Ikebuchi
研究了亚甲蓝和光照射组合对人类免疫缺陷病毒（HIV）1型原代分离株的抗病毒活性，并与其对实验室适应的HIV-1的杀病毒作用进行了比较。在相同病毒原液中，根据逆转录酶活性和病毒RNA评价抗病毒机制。尽管当使用HIV-1HTLV-IIIB作为代表性HIV-1时，在1 μM亚甲蓝和5 J/cm 2照射条件下RNA（>3.07 Log 10）和感染性（6.10 Log 10）显著降低，但观察到病毒包膜（0.20 Log 10）和逆转录酶活性（1.52 Log 10）相对较小的降解。由于在原代分离株和实验室适应性HIV-1（包括HIV-2 ROD）之间未发现感染性降低的差异，因此亚甲蓝光敏化的抗病毒机制可能与所有类型的HIV相似。亚甲蓝光敏化似乎剥夺了HIV的感染性，主要是由于RNA损伤，以及病毒蛋白的弱结构和功能损伤。J. Med. Virol. 62：421-425，2000.© 2000 Wiley‐利斯公司
The antiviral activity for primary isolates of human immunodeficiency virus (HIV) type 1 of a combination of methylene blue and light irradiation was investigated, in comparison with their virucidal effects on laboratory‐adapted HIV‐1. The antiviral mechanism was evaluated in terms of reverse transcriptase activity and viral RNA in the same viral stock. Despite a marked reduction in RNA (>3.07 Log10) and infectivity (6.10 Log10) under conditions of 1 μM methylene blue and 5 J/cm2 irradiation when HIV‐1HTLV‐IIIB as a representative HIV‐1 was employed, relatively little degradation of the viral envelope (0.20 Log10) and reverse transcriptase activity (1.52 Log10) was observed. Because no difference in the reduction of infectivity was found between primary isolates and laboratory‐adapted HIV‐1 (including HIV‐2ROD), the antiviral mechanism of methylene blue photosensitization may be similar for all types of HIVs. Methylene blue photosensitization seems to deprive HIVs of infectivity, mainly due to RNA damage, and weak structural and functional damage of viral proteins. J. Med. Virol. 62:421–425, 2000. © 2000 Wiley‐Liss Inc.
影响因子：
14.9
作者：
SCHNEIDER, JE;PRICE, S;FLOYD, RA
通讯作者：
FLOYD, RA