A combination of insulin and ubiquitin A20 promotes osteocalcin expression in adipose-derived stem cells.
A combination of insulin and ubiquitin A20 promotes osteocalcin expression in adipose-derived stem cells.
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DOI：
10.1139/bcb-2013-0043
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发表时间：
2013-09
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影响因子：
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通讯作者：
Lin Wang;Xia Xu;Na Huo;Huiling Guo;Dongsheng Wang;Hongchen Liu
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文献类型：
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作者：
Lin Wang;Xia Xu;Na Huo;Huiling Guo;Dongsheng Wang;Hongchen Liu
骨细胞生成可用于骨缺损修复；发电效率有待进一步提高。本研究旨在评估泛素A20 （A20）在促进脂肪源性干细胞（ADSCs）骨钙素表达中的作用。在本研究中，从10名健康受试者中获得脂肪组织；从脂肪样品中分离ADSCs。用核心结合因子α -1 （Cbfa1）和/或胰岛素样生长因子-1受体（IGF-1R）转染ADSCs。采用定量RT-PCR （qRT-PCR）和Western blotting检测ADSCs中骨钙素、A20的表达。流式细胞术检测ADSCs的凋亡情况。结果显示，经基因转染和胰岛素刺激后，ADSCs表达高水平骨钙素。然而，IGF-1R激活可诱导ADSCs凋亡。胰岛素可下调ADSCs中Bcl-xL和A20的表达，增加Bax的表达。外源A20的加入抑制了ADSC细胞的凋亡。我们得出结论，IGF-1R的激活可以诱导ADSCs凋亡，这可以通过添加外源性A20来阻止。
Osteocyte generation can be used in bone defect repair; the generation efficiency needs to be further improved. This study aims to evaluate the role of ubiquitin A20 (A20) in facilitating the expression of osteocalcin in adipose-derived stem cells (ADSCs). In this study, adipose tissue was obtained from 10 healthy human subjects; ADSCs were isolated from the adipose samples. The ADSCs were transfected with core binding factor alpha 1 (Cbfa1) and/or insulin-like growth factor-1 receptor (IGF-1R). Expression of osteocalcin, A20 in ADSCs was assessed by quantitative RT-PCR (qRT-PCR) and Western blotting. Apoptosis of ADSCs was analyzed by flow cytometry. The results showed that after the gene transfection and stimulation of insulin, the ADSCs expressed high levels of osteocalcin. However, apoptotic ADSCs were induced by the activation of IGF-1R. Exposure to insulin down-regulated the expression of Bcl-xL and A20, and increased Bax, in ADSCs. The addition of exogenous A20 prevented the ADSC apoptosis. We conclude that activation of IGF-1R can induce apoptosis in ADSCs, which can be prevented by addition of exogenous A20.