Identification, characterization, and developmental regulation of two storage proteins in the bamboo borer Omphisa fuscidentalis.
Identification, characterization, and developmental regulation of two storage proteins in the bamboo borer Omphisa fuscidentalis.
复制标题
DOI：
10.1016/j.jinsphys.2007.08.003
复制
发表时间：
2008
影响因子：
2.2
通讯作者：
Jatuporn Tungjitwitayakul;T. Singtripop;Anchalee Nettagul;Y. Oda;Nujira Tatun;Takayuki Sekimoto;S. Sakurai
中科院分区：
文献类型：
--
作者：
Jatuporn Tungjitwitayakul;T. Singtripop;Anchalee Nettagul;Y. Oda;Nujira Tatun;Takayuki Sekimoto;S. Sakurai
采用三种不同的层析方法，从fuscidentalis幼虫滞育期间的血淋巴中纯化了两个昆虫贮藏蛋白OfSP1 （75kDa）和OfSP2 (72kDa)，并克隆了它们的基因。滞育期间血淋巴中OfSP1和OfSP2浓度较高。在化蛹过程中，雄性血淋巴中OfSP1水平下降，雌性血淋巴中OfSP1水平消失。脂肪体中OfSP1和OfSP2 mRNA水平在第3龄时较低，但在第4、5龄时显著升高。滞育期间mRNA表达量持续低于采食期。向滞育幼虫注射20-羟基蜕皮素（20E）后，注射后2 ~ 3天OfSP1和OfSP2 mRNA水平升高，随后表达下降，直到2 ~ 4天后化蛹。幼体激素类似物（JHA）处理后，OfSP1和OfSP2 mRNA水平逐渐降低，直至化蛹开始。OfSP1和OfSP2蛋白在四龄和五龄幼虫的脂肪体中产生和释放，并在血淋巴中积累，直到昆虫进入滞育。OfSP1可能在滞育结束时被脂肪体重新吸收，以便在化蛹过程中再次使用。
Two insect storage proteins, OfSP1 (75kDa) and OfSP2 (72kDa), were purified using three different chromatographies from the hemolymph of Omphisa fuscidentalis larvae during diapause, and their genes were cloned. OfSP1 and OfSP2 concentrations in the hemolymph were high during diapause. During pupation, OfSP1 levels decreased in the male hemolymph and disappeared from the female hemolymph. OfSP1 and OfSP2 mRNA levels in the fat bodies were low during the third instar, but increased greatly during the fourth and fifth larval instars. During diapause, mRNA expression continued at a lower level than during the feeding period. The injection of 20-hydroxyecdysone (20E) into diapausing larvae caused an increase in OfSP1 and OfSP2 mRNA levels 2−3 days post-injection, followed by a decrease in expression until pupation, which occurred 2−4 days thereafter. When larvae were treated with juvenile-hormone analog (JHA), OfSP1 and OfSP2 mRNA levels gradually decreased until the onset of pupation. In Omphisa, OfSP1 and OfSP2 proteins are produced and released by the larval fat bodies in the fourth and fifth-instar larvae, and the proteins accumulate in the hemolymph until the insects enter diapause. OfSP1 may be reabsorbed by the fat bodies at the end of diapause for subsequent re-use during pupation.