Recombinant bacterial RNA polymerase: Preparation and applications
Recombinant bacterial RNA polymerase: Preparation and applications
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DOI：
10.1016/j.ymeth.2008.10.007
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发表时间：
2009-01-01
期刊：
影响因子：
4.8
通讯作者：
Severinov, Konstantin
中科院分区：
文献类型：
--
作者：
Kuznedelov, Konstantin;Severinov, Konstantin
来自各种细菌的DNA依赖性RNA聚合酶的可用性是建立特定的体外转录系统的关键，所述体外转录系统是理解物种特异性转录调控所必需的。我们在这里描述了两种主要的策略重组RNA聚合酶的纯化-通过体外重建和异源的大肠杆菌中的共同过量生产。这两种策略都可用于从已知rpo（RNA聚合酶）基因序列的任何细菌制备大量RNA聚合酶。(C)2008年爱思唯尔公司All rights reserved.
Availability of DNA-dependent RNA polymerase from various bacteria is a key for setting up specific in vitro transcription systems necessary for understanding species-specific transcription regulation. We describe here two main strategies for recombinant RNA polymerase preparation-through in vitro reconstitution and heterologous co-overproduction in Escherichia coli. Both strategies can be used for preparation of large amounts of RNA polymerases from any bacteria for which sequences of rpo (RNA polymerase) genes are known. (C) 2008 Elsevier Inc. All rights reserved.