Loss-of-Function of a Tomato Receptor-Like Kinase Impairs Male Fertility and Induces Parthenocarpic Fruit Set
Loss-of-Function of a Tomato Receptor-Like Kinase Impairs Male Fertility and Induces Parthenocarpic Fruit Set
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DOI：
10.3389/fpls.2019.00403
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发表时间：
2019-04-16
影响因子：
5.6
通讯作者：
Ariizumi, Tohru
中科院分区：
文献类型：
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作者：
Takei, Hitomi;Shinozaki, Yoshihito;Ariizumi, Tohru
单性结实发生在子房发育成果实而没有授粉/受精时。遗传单性结实的发生机制因其在植物育种中的潜在应用以及对果实早期发育机制的阐明而引起人们的关注。我们已经分离和表征了一种新的小单性结实的果实和花（spff）突变体的番茄（Solanum lycopersicum）品种Micro-Tom。这种植物表现出营养和生殖表型，包括花器官的矮化，雄性不育，延迟开花，改变腋芽发育，单性结实的小果生产。全基因组单核苷酸多态性阵列分析加上定位测序使用下一代测序为基础的高通量方法，导致在一个候选位点的鉴定负责spff突变表型。随后的连锁分析和基于RNA干扰的沉默表明，这些表型是由单一基因（Solyc 04 g 077010）的功能丧失突变引起的，该基因编码一种受体样蛋白激酶，在幼芽的维管束中表达。细胞学和转录组学分析表明，单性结实的spff突变体与卵巢细胞增大和赤霉素代谢基因，GA 20 ox 1的表达升高。两者合计，我们的研究结果表明Solyc 04 g 077010在雄性器官发育中的作用，并表明这种受体样蛋白激酶活性的丧失可能导致单性结实。
Parthenocarpy arises when an ovary develops into fruit without pollination/fertilization. The mechanisms involved in genetic parthenocarpy have attracted attention because of their potential application in plant breeding and also for their elucidation of the mechanisms involved in early fruit development. We have isolated and characterized a novel small parthenocarpic fruit and flower (spff) mutant in the tomato (Solanum lycopersicum) cultivar Micro-Tom. This plant showed both vegetative and reproductive phenotypes including dwarfism of floral organs, male sterility, delayed flowering, altered axillary shoot development, and parthenocarpic production of small fruits. Genome-wide single nucleotide polymorphism array analysis coupled with mapping-by-sequencing using next generation sequencing-based high-throughput approaches resulted in the identification of a candidate locus responsible for the spff mutant phenotype. Subsequent linkage analysis and RNA interference-based silencing indicated that these phenotypes were caused by a loss-of-function mutation of a single gene (Solyc04g077010), which encodes a receptor-like protein kinase that was expressed in vascular bundles in young buds. Cytological and transcriptomic analyses suggested that parthenocarpy in the spff mutant was associated with enlarged ovarian cells and with elevated expression of the gibberellin metabolism gene, GA20ox1. Taken together, our results suggest a role for Solyc04g077010 in male organ development and indicate that loss of this receptor-like protein kinase activity could result in parthenocarpy.