PURIFICATION OF MURINE IGG MONOCLONAL-ANTIBODIES BY PRECIPITATION WITH CAPRYLIC-ACID - COMPARISON WITH OTHER METHODS OF PURIFICATION
PURIFICATION OF MURINE IGG MONOCLONAL-ANTIBODIES BY PRECIPITATION WITH CAPRYLIC-ACID - COMPARISON WITH OTHER METHODS OF PURIFICATION
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DOI：
10.1089/hyb.1989.8.85
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发表时间：
1989-02-01
期刊：
影响因子：
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通讯作者：
FERRONE, S
中科院分区：
文献类型：
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作者：
TEMPONI, M;KAGESHITA, T;FERRONE, S
通过辛酸沉淀从腹水中纯化鼠 IgG 单克隆抗体与以下方法进行比较：1) 通过辛酸和 (NH4)2SO4 连续沉淀；2) 通过抗独特型单克隆抗体或抗小鼠 IgG 异种抗体的亲和层析； 3)依次进行(NH4)2SO4沉淀、离子交换色谱和高压液相色谱(简称HPLC)。就抗体产率而言，辛酸沉淀与辛酸和 (NH4)2SO4 连续沉淀相当，但优于抗体亲和层析和 HPLC。就抗体纯度而言，辛酸沉淀的效率低于其他三种方法。还应注意，辛酸沉淀会降低某些抗体的亲和力，不适合纯化鼠 IgA 和 IgG3。
Purification of murine IgG monoclonal antibodies from ascitic fluid by precipitation with caprylic acid was compared to that: 1) by sequential precipitation with caprylic acid and (NH4)2SO4;2) by affinity chromatography on either antiidiotypic monoclonal antibodies or antimouse IgG xenoantibodies; and 3) by sequential (NH4)2SO4 precipitation, ion exchange chromatography and high pressure liquid chromatography (referred to as HPLC). In terms of yield of antibodies, precipitation with caprylic acid is comparable to sequential precipitation with caprylic acid and (NH4)2SO4, but superior to affinity chromatography on antibodies and to HPLC. In terms of purity of antibodies, precipitation with caprylic acid is less efficient than the other three methods. It should also be noted that precipitation with caprylic acid is associated with a reduction in the affinity of some antibodies and is not suitable to purify murine IgA and IgG3.