Differential Itch- and Pain-related Behavioral Responses and μ-opioid Modulation in Mice
Differential Itch- and Pain-related Behavioral Responses and μ-opioid Modulation in Mice
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DOI：
10.2340/00015555-0962
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发表时间：
2010-01-01
影响因子：
3.6
通讯作者：
Carstens, Earl
中科院分区：
文献类型：
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作者：
Akiyama, Tasuku;Carstens, Mirela Iodi;Carstens, Earl
皮肤内微量注射瘙痒原组胺或辣椒素，分别引起小鼠后肢抓挠或同侧前肢擦拭。我们研究了不同瘙痒原和变应原、类阿片调节剂组胺、5-羟色胺和蛋白酶激活受体激动剂PAR-2和PAR-4引起的后肢抓挠反应的剂量依赖关系。相比之下，辣椒素、辣椒素、异硫氰酸烯丙酯和缓激肽可引起剂量相关的前肢擦拭，吗啡可减少辣椒素引起的前肢擦拭，但不能减少瘙痒原引起的抓挠。La拮抗剂纳曲酮可减少瘙痒引起的抓挠，但不能引起辣椒素引起的擦拭。牛皮针插入真皮内可引起同等程度的抓挠和擦拭，而含有组胺或辣椒素的灭活牛腿针会引起明显更多的抓挠或擦拭。小鼠脸颊注射模型似乎是一种区分瘙痒痛和辣椒素的有用的行为学测试。
Intradermal microinjection of the pruritogen histamine, or the algogen capsaicin, in the mouse cheek differentially elicits mainly hindlimb scratching or ipsilateral forelimb wiping, respectively We investigated the dose-dependency of thew responses elicited by various pruritogens and algogens, and mu-opioid modulation Histamine, 5-hydro-xytryptamine (5-HT) and agonists of protease-activated receptors PAR-2 and PAR-4, all elicited dose-related hindlimb scratching bouts with little forelimb wiping In contrast, capsaicin, allyl isothiocyanate and bradykinin elicited dose-related forelimb wiping with little scratching Morphine reduced capsaicin-evoked wiping but not pruritogen-evoked scratching The la-antagonist naltrexone decreased pruritogen-evoked scratching but not capsaicin-evoked wiping A cowhage spicule inserted intradermal elicited equivalent scratching and wiping, while inactivated cowhage spicules loaded with histamine or capsaicin elicited significantly more scratching or wiping, respectively The mouse cheek injection model appears to be a useful behavioral test that distinguishes between Itch and pain