IMMUNOLOCALIZATION OF PROLIFERATING PERISINUSOIDAL CELLS IN RAT-LIVER
IMMUNOLOCALIZATION OF PROLIFERATING PERISINUSOIDAL CELLS IN RAT-LIVER
复制标题
DOI：
10.1007/bf01082441
复制
发表时间：
1992-02-01
期刊：
影响因子：
--
通讯作者：
BURT, AD
中科院分区：
文献类型：
--
作者：
JOHNSON, SJ;HINES, JE;BURT, AD
现在有大量的证据表明，窦周（伊藤或脂肪储存）细胞是肝纤维化过程中细胞外基质蛋白的主要来源。 在大鼠肝脏中，这些细胞表达中间丝蛋白结蛋白;结蛋白现在被广泛用作这些细胞的免疫组织化学标记。 已经表明，在急性和慢性肝损伤的实验模型中，在基质蛋白沉积之前，结蛋白阳性窦周细胞的数量增加;然而，这些研究未能确定结蛋白阳性窦周细胞群的扩增是否涉及局部增殖。为了研究窦周细胞应答的动力学，我们开发了一种新的双标记免疫组织化学技术，用于在固定石蜡包埋的大鼠肝脏切片中同时显示结蛋白和掺入的溴脱氧尿苷在增殖的窦周细胞中。 应用这种技术的急性肝损伤模型（单剂量四氯化碳灌胃）表明，扩大窦周细胞群是由局部增殖，标记指数为18.7%，2天后损伤。
There is now substantial evidence that perisinusoidal (Ito or fat-storing) cells are the principal source of extracellular matrix proteins during hepatic fibrogenesis. In rat liver these cells express the intermediate filament protein desmin; this is now widely used as an immunohistochemical marker for these cells. It has been shown that in experimental models of acute and chronic liver injury there is an increase in the number of desmin-positive perisinusoidal cells prior to the deposition of matrix proteins; however, these studies have failed to establish whether local proliferation is involved in this expansion of the desmin-positive perisinusoidal cell population.In order to investigate the kinetics of the perisinusoidal cell response, we have developed a novel double-labelling immunohistochemical technique for the simultaneous demonstration of desmin and incorporated bromodeoxyuridine in proliferating perisinusoidal cells in sections of fixed paraffin-embedded rat liver. Application of this technique to a model of acute liver injury (single dose carbon tetrachloride by gavage) has shown that expansion of the perisinusoidal cell population is contributed to by local proliferation, with a labelling index of 18.7% 2 days following injury.