In vivo CD4+ lymph node T cells from lpr mice generate CD4-CD8-B220+TCR-beta low cells.
In vivo CD4+ lymph node T cells from lpr mice generate CD4-CD8-B220+TCR-beta low cells.
复制标题
来自 lpr 小鼠的体内 CD4 淋巴结 T 细胞产生 CD4-CD8-B220 TCR-β 低细胞。
作者：
Y. Laouar;S. Ezine
双阴性CD4-CD8-T细胞（DNT）已被证明是在携带lpr基因的小鼠中导致与狼疮样综合征早期发病相关的大量淋巴结病的主要T细胞群。在此之前，我们证明了这些细胞在它们侵入的外周淋巴器官中不会增殖；此外，我们发现在淋巴结CD4+ T细胞上观察到广泛的CD4 Ag表达。在本研究中，我们使用体内移植系统分析了B6-lpr/lpr小鼠CD4+ T细胞的后代。纯化的CD4+ T细胞注射到B6裸鼠体内，能够生成DNT细胞；此外，体内生成的DNT细胞的表型和功能特征表明，它们与B6-lpr/lpr小鼠的DNT细胞具有相同的特性。我们还表明，在体外溴脱氧尿苷掺入后，只有CD4+细胞周期。从这些研究中，我们得出结论，淋巴细胞增殖发生在CD4+阶段，这种Ag的下调可能伴随着细胞周期的停滞。
Double-negative CD4-CD8-T cells (DNT) have been shown to be the major population of T cells responsible for the massive lymphadenopathy associated with the early onset of the lupus-like syndrome in mice bearing the lpr gene. Previously, we demonstrated that these cells do not proliferate in the peripheral lymphoid organs that they invade; furthermore, we showed that a wide range of CD4 Ag expression was observed on lymph node CD4+ T cells. In this study, we used an in vivo transfer system to analyze the progeny of CD4+ T cells from B6-lpr/lpr mice. Purified CD4+ T cells injected into B6 nude mice are able to generate DNT cells; furthermore, phenotypic and functional characterizations of the DNT cells generated in vivo show that they share the same properties as DNT cells from B6-lpr/lpr mice. We also show that, after in vitro bromodeoxyuridine incorporation, only CD4+ cells cycle. From these studies, we conclude that the lymphoproliferation occurs at the CD4+ stage and that down-regulation of this Ag probably is followed by arrest of the cell cycle.