Selective chemical treatment of cellular microdomains using multiple laminar streams
Selective chemical treatment of cellular microdomains using multiple laminar streams
复制标题
DOI：
10.1016/s1074-5521(03)00019-x
复制
发表时间：
2003-02-01
影响因子：
--
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Takayama, S;Ostuni, E;Whitesides, GM
在许多实验中，用生化试剂处理细胞表面或内部的一部分是有用的。然而，实现亚细胞特异性很困难，因为小分子在几秒钟内扩散的距离等于细胞的范围。本文通过实验演示并从理论上分析，使用微流体通道中的多个层流流体以亚细胞空间选择性将试剂递送至细胞或从细胞中去除试剂。该技术使得荧光标记线粒体的不同亚群、化学破坏细胞骨架的选定区域、酶促去除细胞-基质粘附的有限区域以及观察单个细胞内的微区室内吞作用成为可能。该技术不需要显微注射或将试剂固定到非扩散物体上；它打开了一扇了解细胞生物学的新窗口。
There are many experiments in which it would be useful to treat a part of the surface or interior of a cell with a biochemical reagent. It is difficult, however, to achieve subcellular specificity, because small molecules diffuse distances equal to the extent of the cell in seconds. This paper demonstrates experimentally, and analyzes theoretically, the use of multiple laminar fluid streams in microfluidic channels to deliver reagents to, and remove them from, cells with subcellular spatial selectivity. The technique made it possible to label different subpopulations of mitochondria fluorescently, to disrupt selected regions of the cytoskeleton chemically, to dislodge limited areas of cell-substrate adhesions enzymatically, and to observe microcompartmental endocytosis within individual cells. This technique does not require microinjection or immobilization of reagents onto nondiffusive objects; it opens a new window into cell biology.