CHARACTERIZATION OF THE SACCHAROMYCES GOLGI-COMPLEX THROUGH THE CELL-CYCLE BY IMMUNOELECTRON MICROSCOPY
CHARACTERIZATION OF THE SACCHAROMYCES GOLGI-COMPLEX THROUGH THE CELL-CYCLE BY IMMUNOELECTRON MICROSCOPY
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DOI：
10.1091/mbc.3.7.789
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发表时间：
1992-07-01
影响因子：
3.3
通讯作者：
BOTSTEIN, D
中科院分区：
文献类型：
--
作者：
PREUSS, D;MULHOLLAND, J;BOTSTEIN, D
用免疫电子显微镜对野生型酿酒酵母高尔基体的膜结构进行了鉴定和表征。使用改进的固定方法，通过标记针对α-1-6甘露糖链、Sec7蛋白或Ypt1蛋白的抗体来识别高尔基体间隔。这些抗体标记的间隔看起来像盘状结构，明显被小泡包围。酵母高尔基体通常被视为单一的、孤立的池子，通常不会排列成平行的堆叠。免疫电子显微镜通过酵母细胞周期监测高尔基体结构的位置。在母细胞中总是可以观察到几个明显随机分布的高尔基体隔室。在新的子代细胞的启动过程中，附加的高尔基体结构聚集在萌芽部位的正下方。这些高尔基体在早期进入子细胞，增加了芽的大部分生长可能是由于在女儿体内形成并完全消耗分泌囊泡所致。在胞质分裂过程中，高尔基体集中在细胞壁合成部位附近。芽形成部位和细胞隔膜处高尔基体的聚集表明，这些细胞器可能指向细胞表面快速生长的部位。
The membrane compartments responsible for Golgi functions in wild-type Saccharomyces cerevisiae were identified and characterized by immunoelectron microscopy. Using improved fixation methods, Golgi compartments were identified by labeling with antibodies specific for alpha-1-6 mannose linkages, the Sec7 protein, or the Ypt1 protein. The compartments labeled by each of these antibodies appear as disk-like structures that are apparently surrounded by small vesicles. Yeast Golgi typically are seen as single, isolated cisternae, generally not arranged into parallel stacks. The location of the Golgi structures was monitored by immunoelectron microscopy through the yeast cell cycle. Several Golgi compartments, apparently randomly distributed, were always observed in mother cells. During the initiation of new daughter cells, additional Golgi structures cluster just below the site of bud emergence. These Golgi enter daughter cells at an early stage, raising the possibility that much of the bud's growth might be due to secretory vesicles formed as well as consumed entirely within the daughter. During cytokinesis, the Golgi compartments are concentrated near the site of cell wall synthesis. Clustering of Golgi both at the site of bud formation and at the cell septum suggests that these organelles might be directed toward sites of rapid cell surface growth.