Microwave-assisted extraction of Eucalyptus robusta leaf for the optimal yield of total phenolic compounds
Microwave-assisted extraction of Eucalyptus robusta leaf for the optimal yield of total phenolic compounds
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DOI：
10.1016/j.indcrop.2015.02.044
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发表时间：
2015-07-01
影响因子：
5.9
通讯作者：
Scarlett, Christopher J.
中科院分区：
文献类型：
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作者：
Bhuyan, Deep Jyoti;Quan Van Vuong;Scarlett, Christopher J.
罗布斯塔桉(Eucalyptus robusta，E.robusta)具有重要的传统药用价值，近年来已被证明具有多种体外药理作用。本研究以水为溶剂，采用响应面法(RSM)，以微波辅助提取法(MAE)提取罗布麻中的总酚(TPC)、总黄酮类化合物(TFC)、原花青素含量和抗氧化能力。采用三水平三因素Box-Behnken设计，考察了辐照时间、功率和料液比对这些植物化学物质产率的影响。结果表明，RSM作为一种利用MAE预测TPC、TFC、原花青素和总抗氧化剂产量的工具是准确和可靠的。样品与溶剂的比例对TPC产率的影响最大，其次是功率和辐射时间。TPC和TFC的最佳MAE条件为3min、功率600W、料液比为2g/100mL。在此优化条件下，TPC的实验产率为58.40+/-1.03 mg GAE/g，TFC为19.15+/-1.06 mg RE/g。在这些条件下，TPC产量最高时，原花青素、ABTS、DPPH和CUPRAC值的最大值分别为62%、64.6%、66.3%和67%。本研究表明，MAE是一种从罗布斯塔中高效提取植物化学物质的可靠有效的方法，具有很大的工业、保健或制药应用的扩大潜力。(C)2015爱思唯尔B.V.保留所有权利。
Eucalyptus robusta (E. robusta) has a significant value in traditional medicine and recently has been shown to possess many pharmacological properties in vitro. This study was designed to utilise microwave-assisted extraction (MAE) to yield optimal total phenolic content (TPC), total flavonoid content (TFC), proanthocyanidin levels and antioxidant capacity from E. robusta using water as the solvent, facilitated by the use of response surface methodology (RSM). A three-level-three-factor Box-Behnken design was implemented to elucidate the effect of irradiation time, power and sample-to-solvent ratio on the yields of these phytochemicals. The results highlighted the accuracy and reliability of RSM as a tool for predicting the yields of TPC, TFC, proanthocyanidins and total antioxidants using MAE. Sample-to-solvent ratio had the greatest impact on the TPC yield followed by power and irradiation time. The optimal MAE conditions for TPC and TFC were 3 min, 600 W power and 2 g/100 mL sample-to-solvent ratio. The experimental yield of TPC was 58.40 +/- 1.03 mg GAE/g, and 19.15 +/- 1.06 mg RE/g of TFC was obtained under these optimal conditions. These conditions, optimised for maximum TPC yield also liberated 62%, 64.6%, 66.3% and 67% of the maximum proanthocyanidins, ABTS, DPPH and CUPRAC values, respectively. This study revealed that MAE is a reliable and efficient method for extracting high yields of phytochemicals from E. robusta, with significant potential to be up-scaled for industrial, nutraceutical or pharmaceutical applications. (C) 2015 Elsevier B.V. All rights reserved.