Effect of changing the size of lipid headgroup on peptide insertion into membranes
Effect of changing the size of lipid headgroup on peptide insertion into membranes
复制标题
DOI：
10.1016/s0006-3495(97)78064-0
复制
发表时间：
1997-07-01
影响因子：
3.4
通讯作者：
Huang, HW
中科院分区：
文献类型：
--
作者：
Heller, WT;He, K;Huang, HW
两亲性肽吸附到脂质双层的头基区域是蛋白质-膜相互作用的常见模式。以前的研究表明，吸附导致膜变薄。变薄的程度取决于由肽吸附引起的侧向膨胀的程度。如果这个简单的分子机制是正确的，那么侧向膨胀的程度以及因此而导致的膜变薄的程度应该取决于头基相对于烃链横截面的大小。以前，我们已经建立了丙甲霉素插入过渡和膜变薄效应之间的联系。在本文中，我们使用定向圆二色性来研究不同的头基的大小的影响，同时保持一个恒定的横截面的脂质链上的插入过渡。一个简单的定量预测与实验非常吻合。
Adsorption of amphiphilic peptides to the headgroup region of a lipid bilayer is a common mode of protein-membrane interactions. Previous studies have shown that adsorption causes membrane thinning. The degree of the thinning depends on the degree of the lateral expansion caused by the peptide adsorption. If this simple molecular mechanism is correct, the degree of lateral expansion and consequently the membrane thinning should depend on the size of the headgroup relative to the cross section of the hydrocarbon chains. Previously we have established the connection between the alamethicin insertion transition and the membrane thinning effect. In this paper we use oriented circular dichroism to study the effect of varying the size of the headgroup, while maintaining a constant cross section of the lipid chains, on the insertion transition. A simple quantitative prediction agrees very well with the experiment.